Abstract
We compared a rigid (1-h) screening method for the detection of chloramphenicol acetyltransferase (CAT) activity with the standard spectrophotometric CAT assay to determine whether CAT-mediated chloramphenicol resistance in Haemophilus influenzae could be determined upon primary isolation. Of 58 H. influenzae cell sonicates, 28 had detectable CAT activity when the chloramphenicol-dependent production of free coenzyme A from acetyl coenzyme A was measured spectrophotometrically (standard method). These 28 strains were identified as producing CAT by the rapid method which uses lysed cell suspensions and a color change to detect CAT. The remaining 30 strains did not have CAT activity detectable by either method. This 1-h test for CAT should prove to be useful for the early presumptive identification of chloramphenicol resistance in H. influenzae.
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Selected References
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- Bryan L. E. Transferable chloramphenicol and ampicillin resistance in a strain of Haemophilus influenzae. Antimicrob Agents Chemother. 1978 Jul;14(1):154–156. doi: 10.1128/aac.14.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dietz A. A., Rubinstein H. M. Laboratory note--standardization of the Ellman reaction. Clin Biochem. 1972 Jun;5(2):136–138. doi: 10.1016/s0009-9120(72)80022-5. [DOI] [PubMed] [Google Scholar]
- Kattan S. Letter: Chloramphenicol-resistant Haemophilus. Lancet. 1976 Apr 10;1(7963):814–814. doi: 10.1016/s0140-6736(76)91663-9. [DOI] [PubMed] [Google Scholar]