Abstract
An anion-exchange column technique for extraction of antibiotics from serum proteins has been developed for use in the assay of cephapirin, cefotaxime, and cefoxitin by high-pressure liquid chromatography. Anion-exchange extraction of cephapirin from serum samples by this technique was compared with protein precipitation methods, using 6% trichloroacetic acid or absolute ethanol. Column extraction gave improved quantitative drug recovery and reduced background serum interferences in the resultant chromatograms when evaluated against protein precipitation. Comparisons of this method with microbiological assay gave statistically equivalent results. Twelve patient samples were assayed for cephapirin, and no interferences were encountered from the 22 systemic agents these subjects were receiving. The anion-exchange technique for antibiotic extraction provides a rapid, precise, and quantitative antibiotic assay when used with liquid chromatography.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anhalt J. P. Assay of gentamicin in serum by high-pressure liquid chromatography. Antimicrob Agents Chemother. 1977 Apr;11(4):651–655. doi: 10.1128/aac.11.4.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bergan T., Solberg R. Assay of cefotaxime by high-pressure-liquid chromatography. Chemotherapy. 1981;27(3):155–165. doi: 10.1159/000237972. [DOI] [PubMed] [Google Scholar]
- Nightingale C. H., Greene D. S., Quintiliani R. Pharmacokinetics and clinical use of cephalosporin antibiotics. J Pharm Sci. 1975 Dec;64(12):1899–1926. doi: 10.1002/jps.2600641202. [DOI] [PubMed] [Google Scholar]
- Sabath L. D. The assay of antimicrobial compounds. Hum Pathol. 1976 May;7(3):287–295. doi: 10.1016/s0046-8177(76)80039-1. [DOI] [PubMed] [Google Scholar]
- Torchia M. G., Danzinger R. G. Reversed-phase high-performance liquid chromatographic assay for cefoxitin in proteinaceous biological samples. J Chromatogr. 1980 Jan 11;181(1):120–122. doi: 10.1016/s0378-4347(00)81279-7. [DOI] [PubMed] [Google Scholar]
- Yoshikawa T. T., Maitra S. K., Schotz M. C., Guze L. B. High-pressure liquid chromatography for quantitation of antimicrobial agents. Rev Infect Dis. 1980 Mar-Apr;2(2):169–181. doi: 10.1093/clinids/2.2.169. [DOI] [PubMed] [Google Scholar]