Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1982 May;21(5):844–845. doi: 10.1128/aac.21.5.844

Effect of dihydrostreptomycin on active transport in isolated bacterial membrane vesicles.

R G Eagon, R J Hartle, J B Rake, S Abdel-Sayed
PMCID: PMC182025  PMID: 6179466

Abstract

Membrane vesicles prepared from bacterial cells grown in the absence of dihydrostreptomycin but subsequently incubated in the presence of dihydrostreptomycin transported proline normally, but vesicles prepared from cells grown in media to which dihydrostreptomycin was added 30 min before harvesting had a greatly impaired ability to accumulate proline. The latter cells extruded protons normally but were unable to maintain a proton gradient as effectively as normal cells. These data indicated that metabolism was required for dihydrostreptomycin to exert an effect on the bacterial cell membrane.

Full text

PDF
844

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANAND N., DAVIS B. D. Damage by streptomycin to the cell membrane of Escherichia coli. Nature. 1960 Jan 2;185:22–23. doi: 10.1038/185022a0. [DOI] [PubMed] [Google Scholar]
  2. Bryan L. E., Van den Elzen H. M. Streptomycin accumulation in susceptible and resistant strains of Escherichia coli and Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1976 Jun;9(6):928–938. doi: 10.1128/aac.9.6.928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUBIN D. T., HANCOCK R., DAVIS B. D. THE SEQUENCE OF SOME EFFECTS OF STREPTOMYCIN IN ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Aug 13;74:476–489. doi: 10.1016/0006-3002(63)91390-8. [DOI] [PubMed] [Google Scholar]
  4. Hancock R. E., Raffle V. J., Nicas T. I. Involvement of the outer membrane in gentamicin and streptomycin uptake and killing in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1981 May;19(5):777–785. doi: 10.1128/aac.19.5.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaback H. R. Transport in isolated bacterial membrane vesicles. Methods Enzymol. 1974;31:698–709. doi: 10.1016/0076-6879(74)31075-0. [DOI] [PubMed] [Google Scholar]
  6. Rake J. B., Eagon R. G. Inhibition, but not uncoupling, of respiratory energy coupling of three bacterial species by nitrite. J Bacteriol. 1980 Dec;144(3):975–982. doi: 10.1128/jb.144.3.975-982.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES