Abstract
Enrichment cultures containing atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) at a concentration of 100 ppm (0.46 mM) as a sole nitrogen source were obtained from soils exposed to repeated spills of atrazine, alachlor, and metolachlor. Bacterial growth occurred concomitantly with formation of metabolites from atrazine and subsequent biosynthesis of protein. When ring-labeled [14C]atrazine was used, 80% or more of the s-triazine ring carbon atoms were liberated as 14CO2. Hydroxyatrazine may be an intermediate in the atrazine mineralization pathway. More than 200 pure cultures isolated from the enrichment cultures failed to utilize atrazine as a nitrogen source. Mixing pure cultures restored atrazine-mineralizing activity. Repeated transfer of the mixed cultures led to increased rates of atrazine metabolism. The rate of atrazine degradation, even at the elevated concentrations used, far exceeded the rates previously reported in soils, waters, and mixed and pure cultures of bacteria.
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Selected References
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- Frank R., Sirons G. J. Dissipation of atrazine residues from soils. Bull Environ Contam Toxicol. 1985 Apr;34(4):541–548. doi: 10.1007/BF01609773. [DOI] [PubMed] [Google Scholar]
- Geller A. Studies on the degradation of atrazine by bacterial communities enriched from various biotopes. Arch Environ Contam Toxicol. 1980;9(3):289–305. doi: 10.1007/BF01057409. [DOI] [PubMed] [Google Scholar]
- Holden D. W., Kronstad J. W., Leong S. A. Mutation in a heat-regulated hsp70 gene of Ustilago maydis. EMBO J. 1989 Jul;8(7):1927–1934. doi: 10.1002/j.1460-2075.1989.tb03596.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jessee J. A., Benoit R. E., Hendricks A. C., Allen G. C., Neal J. L. Anaerobic degradation of cyanuric Acid, cysteine, and atrazine by a facultative anaerobic bacterium. Appl Environ Microbiol. 1983 Jan;45(1):97–102. doi: 10.1128/aem.45.1.97-102.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]