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. 1993 Jul;59(7):2107–2111. doi: 10.1128/aem.59.7.2107-2111.1993

Purification and partial characterization of an elastolytic serine protease of Prevotella intermedia.

Y Shibata 1, S Fujimura 1, T Nakamura 1
PMCID: PMC182243  PMID: 8357246

Abstract

Elastolytic strains of Prevotella intermedia were isolated from pus samples of adult periodontal lesions. Elastase was found to associate with envelope, and it could be solubilized with guanidine-HCl. The enzyme was purified to homogeneity by sequential procedures including ion-exchange chromatography, gel filtration, and hydrophobic interaction chromatography. This elastase was a serine protease, and its mass was 31 kDa. It hydrolyzed elastin powder, but collagen and azodye-conjugated proteins were not degraded by this enzyme. Both synthetic substrates for human pancreatic (glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucine p-nitroanilide) and leukocyte elastase (methoxy succinyl-L-alanyl-alanyl-L-prolyl-L-valine p-nitroanilide) were hydrolyzed.

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Selected References

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