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. 1993 Aug;59(8):2589–2593. doi: 10.1128/aem.59.8.2589-2593.1993

Influence of temperature and relative humidity on the survival of Chlamydia pneumoniae in aerosols.

H J Theunissen 1, N A Lemmens-den Toom 1, A Burggraaf 1, E Stolz 1, M F Michel 1
PMCID: PMC182324  PMID: 8368846

Abstract

The survival of Chlamydia pneumoniae in aerosols was investigated by using a chamber with a capacity of 114.5 liters. We injected 5 x 10(7) inclusion-forming units (IFU) of C. pneumoniae in aerosols with a droplet size of 3 to 5 microns. Samples were taken after 30 s and every 1 min thereafter. The survival of C. pneumoniae was measured at four temperatures (8.5, 15, 25, and 35 degrees C) and at three different relative humidities (RH) of 5, 50, and 95% for each temperature. The survival rates of Streptococcus pneumoniae, Streptococcus faecalis, Klebsiella pneumoniae, Chlamydia trachomatis LGV2, and cytomegalovirus were also determined at 25 degrees C and 95% RH and compared with that of C. pneumoniae. At the mentioned temperatures and RH, a rapid decrease of C. pneumoniae IFU was observed in the first 30 s. After this the decrease in the number of IFU was more gradual. The survival of C. pneumoniae in aerosols were optimal at 15 to 25 degrees C and 95% RH; it was good compared with those of other microorganisms. A lower death rate was observed only in S. faecalis. In C. trachomatis, the death rate during the first 30 s was higher than that in C. pneumoniae (85 and 53.3%, respectively). After the first 30 s, the death rates in the two organisms were identical. It was concluded that transmission of C. pneumoniae via aerosols was possible. There is probably a direct transmission from person to person, taking into account the relatively short survival period of C. pneumoniae in aerosols.

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Selected References

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  1. Bavoil P., Ohlin A., Schachter J. Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis. Infect Immun. 1984 May;44(2):479–485. doi: 10.1128/iai.44.2.479-485.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cles L. D., Stamm W. E. Use of HL cells for improved isolation and passage of Chlamydia pneumoniae. J Clin Microbiol. 1990 May;28(5):938–940. doi: 10.1128/jcm.28.5.938-940.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cox C. S. Airborne bacteria and viruses. Sci Prog. 1989;73(292 Pt 4):469–499. [PubMed] [Google Scholar]
  4. Cox C. S. The survival of Escherichia coli sprayed into air and into nitrogen from distilled water and from solutions of protecting agents, as a function of relative humidity. J Gen Microbiol. 1966 Jun;43(3):383–399. doi: 10.1099/00221287-43-3-383. [DOI] [PubMed] [Google Scholar]
  5. Eze M. O., McElhaney R. N. The effect of alterations in the fluidity and phase state of the membrane lipids on the passive permeation and facilitated diffusion of glycerol in Escherichia coli. J Gen Microbiol. 1981 Jun;124(2):299–307. doi: 10.1099/00221287-124-2-299. [DOI] [PubMed] [Google Scholar]
  6. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  7. Grayston J. T., Wang S. P., Kuo C. C., Campbell L. A. Current knowledge on Chlamydia pneumoniae, strain TWAR, an important cause of pneumonia and other acute respiratory diseases. Eur J Clin Microbiol Infect Dis. 1989 Mar;8(3):191–202. doi: 10.1007/BF01965260. [DOI] [PubMed] [Google Scholar]
  8. Hatch M. T., Dimmick R. L. Physiological responses of airborne bacteria to shifts in relative humidity. Bacteriol Rev. 1966 Sep;30(3):597–603. doi: 10.1128/br.30.3.597-603.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hyman C. L., Augenbraun M. H., Roblin P. M., Schachter J., Hammerschlag M. R. Asymptomatic respiratory tract infection with Chlamydia pneumoniae TWAR. J Clin Microbiol. 1991 Sep;29(9):2082–2083. doi: 10.1128/jcm.29.9.2082-2083.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jenkin H. M. Comparative lipid composition of psittacosis and trachoma agents. Am J Ophthalmol. 1967 May;63(5 Suppl):1087–1098. doi: 10.1016/0002-9394(67)94087-1. [DOI] [PubMed] [Google Scholar]
  11. Songer J. R. Influence of relative humidity on the survival of some airborne viruses. Appl Microbiol. 1967 Jan;15(1):35–42. doi: 10.1128/am.15.1.35-42.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Theunissen J. J., van Heijst B. Y., Wagenvoort J. H., Stolz E., Michel M. F. Factors influencing the infectivity of Chlamydia pneumoniae elementary bodies on HL cells. J Clin Microbiol. 1992 Jun;30(6):1388–1391. doi: 10.1128/jcm.30.6.1388-1391.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WEBB S. J. Factors affecting the viability of air-borne bacteria. II. The effect of chemical additives on the behavior of air-borne cells. Can J Microbiol. 1960 Feb;6:71–87. doi: 10.1139/m60-010. [DOI] [PubMed] [Google Scholar]
  14. de Jong J. C., Winkler K. C. The inactivation of poliovirus in aerosols. J Hyg (Lond) 1968 Dec;66(4):557–565. doi: 10.1017/s0022172400028308. [DOI] [PMC free article] [PubMed] [Google Scholar]

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