Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Aug;59(8):2673–2677. doi: 10.1128/aem.59.8.2673-2677.1993

Production of [14C]fumonisin B1 by Fusarium moniliforme MRC 826 in corn cultures.

J F Alberts 1, W C Gelderblom 1, R Vleggaar 1, W F Marasas 1, J P Rheeder 1
PMCID: PMC182337  PMID: 8368853

Abstract

Kinetics of growth and fumonisin production by Fusarium moniliforme MRC 826 in corn "patty" cultures were investigated, and a technique was developed for the production of [14C]fumonisin B1 ([14C]FB1) by using L-[methyl-14C]methionine as the precursor. A significant (P < 0.01) correlation exists between fungal growth and FB1 (r = 0.89) and FB2 (r = 0.87) production in corn patties, beginning after 2 days and reaching the stationary phase after 14 days of incubation. [14C]FB1 was produced by adding L-[methyl-14C]methionine daily to cultures during the logarithmic phase of production. Incorporation of the isotope occurred at C-21 and C-22 of the fumonism molecule and was enhanced in the presence of unlabeled L-methionine. Although the concentration of exogenous unlabeled methionine is critical for incorporation of the 14C label, optimum incorporation was achieved by adding 50 mg of unlabeled L-methionine and 200 mu Ci of L-[methyl-14C]methionine to a corn patty (30 g) over a period of 9 days, yielding [14C]FB1 with a specific activity of 36 mu Ci/mmol.

Full text

PDF
2673

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts J. F., Gelderblom W. C., Thiel P. G., Marasas W. F., Van Schalkwyk D. J., Behrend Y. Effects of temperature and incubation period on production of fumonisin B1 by Fusarium moniliforme. Appl Environ Microbiol. 1990 Jun;56(6):1729–1733. doi: 10.1128/aem.56.6.1729-1733.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appelgren L. E., Arora R. G., Larsson P. Autoradiographic studies of [3H]zearalenone in mice. Toxicology. 1982;25(2-3):243–253. doi: 10.1016/0300-483x(82)90033-6. [DOI] [PubMed] [Google Scholar]
  3. Blackwell B. A., Miller J. D., Greenhalgh R. 13C NMR study of the biosynthesis of toxins by Fusarium graminearum. J Biol Chem. 1985 Apr 10;260(7):4243–4247. [PubMed] [Google Scholar]
  4. Chi M. S., Robison T. S., Mirocha C. J., Swanson S. P., Shimoda W. Excretion and tissue distribution of radioactivity from tritium-labeled T-2 toxin in chicks. Toxicol Appl Pharmacol. 1978 Aug;45(2):391–402. doi: 10.1016/0041-008x(78)90103-5. [DOI] [PubMed] [Google Scholar]
  5. Dailey R. E., Reese R. E., Brouwer E. A. Metabolism of [14C]zearalenone in laying hens. J Agric Food Chem. 1980 Mar-Apr;28(2):286–291. doi: 10.1021/jf60228a008. [DOI] [PubMed] [Google Scholar]
  6. Gelderblom W. C., Kriek N. P., Marasas W. F., Thiel P. G. Toxicity and carcinogenicity of the Fusarium moniliforme metabolite, fumonisin B1, in rats. Carcinogenesis. 1991 Jul;12(7):1247–1251. doi: 10.1093/carcin/12.7.1247. [DOI] [PubMed] [Google Scholar]
  7. Hagler W. M., Mirocha C. J. Biosynthesis of [14C]zearalenone from [1-14C]acetate by Fusarium roseum 'Gibbosum'. Appl Environ Microbiol. 1980 Mar;39(3):668–670. doi: 10.1128/aem.39.3.668-670.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hagler W. M., Mirocha C. J., Pathre S. V. Biosynthesis of radiolabeled T-2 toxin by Fusarium tricinctum. Appl Environ Microbiol. 1981 Apr;41(4):1049–1051. doi: 10.1128/aem.41.4.1049-1051.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harrison L. R., Colvin B. M., Greene J. T., Newman L. E., Cole J. R., Jr Pulmonary edema and hydrothorax in swine produced by fumonisin B1, a toxic metabolite of Fusarium moniliforme. J Vet Diagn Invest. 1990 Jul;2(3):217–221. doi: 10.1177/104063879000200312. [DOI] [PubMed] [Google Scholar]
  10. Jackson M. A., Bennett G. A. Production of fumonisin B1 by Fusarium moniliforme NRRL 13616 in submerged culture. Appl Environ Microbiol. 1990 Aug;56(8):2296–2298. doi: 10.1128/aem.56.8.2296-2298.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kellerman T. S., Marasas W. F., Thiel P. G., Gelderblom W. C., Cawood M., Coetzer J. A. Leukoencephalomalacia in two horses induced by oral dosing of fumonisin B1. Onderstepoort J Vet Res. 1990 Dec;57(4):269–275. [PubMed] [Google Scholar]
  12. Mantle P. G., Shipston N. F. Temporal separation of steps in the biosynthesis of verruculogen. Biochem Int. 1987 Jun;14(6):1115–1120. [PubMed] [Google Scholar]
  13. Mirocha C. J., Robison T. S., Pawlosky R. J., Allen N. K. Distribution and residue determination of [3H]zearalenone in broilers. Toxicol Appl Pharmacol. 1982 Oct;66(1):77–87. doi: 10.1016/0041-008x(82)90062-x. [DOI] [PubMed] [Google Scholar]
  14. Pace J. G., Watts M. R. Hepatic subcellular distribution of [3H]T-2 toxin. Toxicon. 1989;27(12):1307–1311. doi: 10.1016/0041-0101(89)90062-7. [DOI] [PubMed] [Google Scholar]
  15. Pathre S. V., Khadikar P. V., Mirocha C. J. Biosynthesis of zearalenone: a simple and efficient method to incorporate [13C]acetate label by using solid cultures. Appl Environ Microbiol. 1989 Aug;55(8):1955–1956. doi: 10.1128/aem.55.8.1955-1956.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Plattner R. D., Shackelford D. D. Biosynthesis of labeled fumonisins in liquid cultures of Fusarium moniliforme. Mycopathologia. 1992 Feb;117(1-2):17–22. doi: 10.1007/BF00497274. [DOI] [PubMed] [Google Scholar]
  17. Shephard G. S., Thiel P. G., Sydenham E. W., Alberts J. F., Gelderblom W. C. Fate of a single dose of the 14C-labelled mycotoxin, fumonisin B1, in rats. Toxicon. 1992 Jul;30(7):768–770. doi: 10.1016/0041-0101(92)90011-s. [DOI] [PubMed] [Google Scholar]
  18. Shephard G. S., Thiel P. G., Sydenham E. W. Initial studies on the toxicokinetics of fumonisin B1 in rats. Food Chem Toxicol. 1992 Apr;30(4):277–279. doi: 10.1016/0278-6915(92)90004-5. [DOI] [PubMed] [Google Scholar]
  19. Yoshizawa T., Mirocha C. J., Behrens J. C., Swanson S. P. Metabolic fate of T-2 toxin in a lactating cow. Food Cosmet Toxicol. 1981 Feb;19(1):31–39. doi: 10.1016/0015-6264(81)90300-x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES