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. 1993 Sep;59(9):2777–2783. doi: 10.1128/aem.59.9.2777-2783.1993

Degradation of 2-Chloroethylvinylether by Ancylobacter aquaticus AD25 and AD27

Arjan J van den Wijngaard 1, Janke Prins 1, Arjan J A C Smal 1, Dick B Janssen 1,*
PMCID: PMC182365  PMID: 16349032

Abstract

Incubation of five different β-chloroethers with slurries prepared from brackish water sediment or activated sludge revealed that bis(2-chloroethyl)ether and 2-chloroethylvinylether (2-CVE) were biodegradable under aerobic conditions. After enrichment, two different cultures of Ancylobacter aquaticus that are capable of growth on 2-CVE were isolated. Both cultures were also able to grow on 1,2-dichloroethane. The cells contained a haloalkane dehalogenase that dehalogenated 2-CVE, 2-chloroethylmethylether, 2-bromoethylethylether, and epichlorohydrin. Experiments with cell extracts indicated that an alcohol dehydrogenase and an aldehyde dehydrogenase were also involved in the degradation of 2-CVE. This suggests that 2-CVE is metabolized via 2-hydroxyethylvinylether and vinyloxyacetaldehyde to vinyloxyacetic acid. Enzymatic ether cleavage was not detected. 2-CVE was also degraded by chemical ether cleavage, leading to the formation of 2-chloroethanol and acetaldehyde, both of which supported growth. We propose that A. aquaticus strains may be important for the detoxification and degradation of halogenated aliphatic compounds in the environment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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