Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Sep;59(9):2914–2917. doi: 10.1128/aem.59.9.2914-2917.1993

Biological inactivation of adhering Listeria monocytogenes by listeriaphages and a quaternary ammonium compound.

B Roy 1, H W Ackermann 1, S Pandian 1, G Picard 1, J Goulet 1
PMCID: PMC182386  PMID: 8215364

Abstract

The use of listeriaphages as a means of disinfecting contaminated stainless-steel and polypropylene surfaces was investigated. Surfaces artificially contaminated with L. monocytogenes 10401 and 8427 were sanitized with suspensions of listeriaphages (H387, H387-A, and 2671), all belonging to the Siphoviridae family. Phage suspensions at concentrations of up to 3.5 x 10(8) PFU/ml were at least as efficient as a 20 ppm solution of a quaternary ammonium compound (QUATAL) in reducing L. monocytogenes populations. A synergistic activity was observed when two or more phages were used in combination and when phages were suspended in QUATAL. The biological activity of the three phages was not affected by QUATAL concentrations of 50 ppm and a contact time of 4 h.

Full text

PDF
2914

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Busscher H. J., Weerkamp A. H., van der Mei H. C., van Pelt A. W., de Jong H. P., Arends J. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl Environ Microbiol. 1984 Nov;48(5):980–983. doi: 10.1128/aem.48.5.980-983.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Costerton J. W., Geesey G. G., Cheng K. J. How bacteria stick. Sci Am. 1978 Jan;238(1):86–95. doi: 10.1038/scientificamerican0178-86. [DOI] [PubMed] [Google Scholar]
  3. Farber J. M., Peterkin P. I. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991 Sep;55(3):476–511. doi: 10.1128/mr.55.3.476-511.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gellin B. G., Broome C. V. Listeriosis. JAMA. 1989 Mar 3;261(9):1313–1320. [PubMed] [Google Scholar]
  5. Gélinas P., Goulet J. Efficacité de huit désinfectants sur trois types de surfaces contaminées par Pseudomonas aeruginosa. Can J Microbiol. 1983 Dec;29(12):1715–1730. [PubMed] [Google Scholar]
  6. Loessner M. J., Busse M. Bacteriophage typing of Listeria species. Appl Environ Microbiol. 1990 Jun;56(6):1912–1918. doi: 10.1128/aem.56.6.1912-1918.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Macpherson E. E., Montagna W. Proceedings: The mammary glands of rhesus monkeys. J Invest Dermatol. 1974 Jul;63(1):17–18. doi: 10.1111/1523-1747.ep12677294. [DOI] [PubMed] [Google Scholar]
  8. Mafu A. A., Roy D., Goulet J., Savoie L. Characterization of physicochemical forces involved in adhesion of Listeria monocytogenes to surfaces. Appl Environ Microbiol. 1991 Jul;57(7):1969–1973. doi: 10.1128/aem.57.7.1969-1973.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ortel S., Ackermann H. W. Morphologie von neuen Listeria-Phagen. Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Dec;260(4):423–427. [PubMed] [Google Scholar]
  10. ROGERS M. R., MAHER J. T., KAPLAN A. M. A practical approach to evaluation of the germicidal efficiency of a general purpose military disinfectant. Appl Microbiol. 1961 Nov;9:497–501. doi: 10.1128/am.9.6.497-501.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STEDMAN R. L., KRAVITZ E., BELL H. Studies on the efficiencies of disinfectants for use on inanimate objects. II. Relative activities on porous surfaces. Appl Microbiol. 1954 Nov;2(6):322–325. doi: 10.1128/am.2.6.322-325.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zink R., Loessner M. J. Classification of virulent and temperate bacteriophages of Listeria spp. on the basis of morphology and protein analysis. Appl Environ Microbiol. 1992 Jan;58(1):296–302. doi: 10.1128/aem.58.1.296-302.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES