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. 1993 Oct;59(10):3280–3286. doi: 10.1128/aem.59.10.3280-3286.1993

Attachment Stimulates Exopolysaccharide Synthesis by a Bacterium

Philippe Vandevivere 1, David L Kirchman 1,*
PMCID: PMC182449  PMID: 16349064

Abstract

This study examined the hypothesis that solid surfaces may stimulate attached bacteria to produce exopolymers. Addition of sand to shake-flask cultures seemed to induce exopolymer synthesis by a number of subsurface isolates, as revealed by optical microscopy. Several additional lines of evidence indicated that exopolymer production by attached cells (in continuous-flow sand-packed columns) was greater than by their free-living counterparts. Total carbohydrates and extracellular polysaccharides, both normalized to cell protein, were greater (2.5- and 5-fold, respectively) for attached cells than for free-living cells. Also, adsorption of a polyanion-binding dye to the exopolymer fraction was sixfold greater for attached cells than for unattached cells. When surface-grown cells were resuspended in fresh medium, exopolymer production decreased to the level characteristic of unattached cells, which ruled out the possibility that attached cells comprised a subpopulation of sticky mucoid variants. The mechanism by which attachment stimulated exopolymer synthesis did not involve changes of the specific growth rate, growth stage, or limiting nutrient.

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Selected References

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