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. 1993 Oct;59(10):3485–3487. doi: 10.1128/aem.59.10.3485-3487.1993

Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.

A Villaverde 1, A Benito 1, E Viaplana 1, R Cubarsi 1
PMCID: PMC182479  PMID: 8250569

Abstract

The expression at different temperatures of the lacZ gene, which is controlled by the lambda pL and pR tandem promoters and the cI857 temperature-sensitive repressor, was studied in Escherichia coli continuous cultures. At temperatures between 30 and 42 degrees C, beta-galactosidase activity behaved according to an exponential equation. By inducing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.

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Selected References

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