Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Nov;59(11):3674–3680. doi: 10.1128/aem.59.11.3674-3680.1993

Comparison of Giardia lamblia and Giardia muris cyst inactivation by ozone.

G R Finch 1, E K Black 1, C W Labatiuk 1, L Gyürék 1, M Belosevic 1
PMCID: PMC182516  PMID: 8285675

Abstract

Inactivation of Giardia lamblia and Giardia muris cysts was compared by using an ozone demand-free 0.05 M phosphate buffer in bench-scale batch reactors at 22 degrees C. Ozone was added to each trial from a concentrated stock solution for contact times of 2 and 5 min. The viability of the control and treated cysts was evaluated by using the C3H/HeN mouse and Mongolian gerbil models for G. muris and G. lamblia, respectively. The resistance of G. lamblia to ozone was not significantly different from that of G. muris under the study conditions, contrary to previously reported data that suggested G. lamblia was significantly more sensitive to ozone than G. muris was. The simple Ct value for 2 log unit inactivation of G. lamblia was 2.4 times higher than the Ct value recommended by the Surface Water Treatment Rule.

Full text

PDF
3674

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belosevic M., Faubert G. M. Giardia muris: correlation between oral dosage, course of infection, and trophozoite distribution in the mouse small intestine. Exp Parasitol. 1983 Aug;56(1):93–100. doi: 10.1016/0014-4894(83)90100-5. [DOI] [PubMed] [Google Scholar]
  2. Belosevic M., Faubert G. M., MacLean J. D., Law C., Croll N. A. Giardia lamblia infections in Mongolian gerbils: an animal model. J Infect Dis. 1983 Feb;147(2):222–226. doi: 10.1093/infdis/147.2.222. [DOI] [PubMed] [Google Scholar]
  3. Belosevic M., Faubert G. M., Skamene E., MacLean J. D. Susceptibility and resistance of inbred mice to Giardia muris. Infect Immun. 1984 May;44(2):282–286. doi: 10.1128/iai.44.2.282-286.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Belosevic M., Faubert G. M. Temporal study of acquired resistance in infections of mice with Giardia muris. Parasitology. 1983 Dec;87(Pt 3):517–524. doi: 10.1017/s0031182000083037. [DOI] [PubMed] [Google Scholar]
  5. Burleson G. R., Murray T. M., Pollard M. Inactivation of viruses and bacteria by ozone, with and without sonication. Appl Microbiol. 1975 Mar;29(3):340–344. doi: 10.1128/am.29.3.340-344.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Faubert G. M., Belosevic M., Walker T. S., MacLean J. D., Meerovitch E. Comparative studies on the pattern of infection with Giardia spp. in mongolian gerbils. J Parasitol. 1983 Oct;69(5):802–805. [PubMed] [Google Scholar]
  7. Finch G. R., Stiles M. E., Smith D. W. Recovery of a marker strain of Escherichia coli from ozonated water by membrane filtration. Appl Environ Microbiol. 1987 Dec;53(12):2894–2896. doi: 10.1128/aem.53.12.2894-2896.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Labatiuk C. W., Schaefer F. W., 3rd, Finch G. R., Belosevic M. Comparison of animal infectivity, excystation, and fluorogenic dye as measures of Giardia muris cyst inactivation by ozone. Appl Environ Microbiol. 1991 Nov;57(11):3187–3192. doi: 10.1128/aem.57.11.3187-3192.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roberts-Thomson I. C., Stevens D. P., Mahmoud A. A., Warren K. S. Acquired resistance to infection in an animal model of giardiasis. J Immunol. 1976 Nov;117(5 PT2):2036–2037. [PubMed] [Google Scholar]
  10. Roberts-Thomson I. C., Stevens D. P., Mahmoud A. A., Warren K. S. Giardiasis in the mouse: an animal model. Gastroenterology. 1976 Jul;71(1):57–61. [PubMed] [Google Scholar]
  11. Schaefer F. W., 3rd, Johnson C. H., Hsu C. H., Rice E. W. Determination of Giardia lamblia cyst infective dose for the Mongolian gerbil (Meriones unguiculatus). Appl Environ Microbiol. 1991 Aug;57(8):2408–2409. doi: 10.1128/aem.57.8.2408-2409.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Visvesvara G. S., Dickerson J. W., Healy G. R. Variable infectivity of human-derived Giardia lamblia cysts for Mongolian gerbils (Meriones unguiculatus). J Clin Microbiol. 1988 May;26(5):837–841. doi: 10.1128/jcm.26.5.837-841.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wickramanayake G. B., Rubin A. J., Sproul O. J. Inactivation of Giardia lamblia cysts with ozone. Appl Environ Microbiol. 1984 Sep;48(3):671–672. doi: 10.1128/aem.48.3.671-672.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES