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. 1991 Jan;57(1):307–310. doi: 10.1128/aem.57.1.307-310.1991

Analysis of Dictyostelium mucoroides Macrocysts by Using a Simple Breakage and Fractionation Procedure

Michael J North 1,*, David A Cotter 1
PMCID: PMC182703  PMID: 16348399

Abstract

A method is described for the breakage and fractionation of Dictyostelium macrocysts. It involves vortexing with large glass beads to disrupt the macrocyst wall followed by differential low-speed centrifugation. It yields fractions containing purified wall material and intact endocytes. The endocytes can themselves be disrupted by using smaller glass beads.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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