Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Jan;57(1):324–326. doi: 10.1128/aem.57.1.324-326.1991

Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components.

A Elsner 1, F Löffler 1, K Miyashita 1, R Müller 1, F Lingens 1
PMCID: PMC182707  PMID: 2036019

Abstract

Extracts of Pseudomonas sp. strain CBS3 grown with 4-chlorobenzoate as sole carbon source contained an enzyme that converted 4-chlorobenzoate to 4-hydroxybenzoate. This enzyme was shown to consist of three components, all necessary for the reaction. Component I, which had a molecular weight of about 3,000, was highly unstable. Components II and III were stable proteins with molecular weights of about 86,000 and 92,000.

Full text

PDF
324

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adriaens P., Kohler H. P., Kohler-Staub D., Focht D. D. Bacterial dehalogenation of chlorobenzoates and coculture biodegradation of 4,4'-dichlorobiphenyl. Appl Environ Microbiol. 1989 Apr;55(4):887–892. doi: 10.1128/aem.55.4.887-892.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fetzner S., Müller R., Lingens F. Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS. Biol Chem Hoppe Seyler. 1989 Nov;370(11):1173–1182. doi: 10.1515/bchm3.1989.370.2.1173. [DOI] [PubMed] [Google Scholar]
  3. Marks T. S., Smith A. R., Quirk A. V. Degradation of 4-Chlorobenzoic Acid by Arthrobacter sp. Appl Environ Microbiol. 1984 Nov;48(5):1020–1025. doi: 10.1128/aem.48.5.1020-1025.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Marks T. S., Wait R., Smith A. R., Quirk A. V. The origin of the oxygen incorporated during the dehalogenation/hydroxylation of 4-chlorobenzoate by an Arthrobacter sp. Biochem Biophys Res Commun. 1984 Oct 30;124(2):669–674. doi: 10.1016/0006-291x(84)91607-3. [DOI] [PubMed] [Google Scholar]
  5. Markus A., Klages U., Krauss S., Lingens F. Oxidation and dehalogenation of 4-chlorophenylacetate by a two-component enzyme system from Pseudomonas sp. strain CBS3. J Bacteriol. 1984 Nov;160(2):618–621. doi: 10.1128/jb.160.2.618-621.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Müller R., Oltmanns R. H., Lingens F. Enzymatic dehalogenation of 4-chlorobenzoate by extracts from Arthrobacter sp. SU DSM 20407. Biol Chem Hoppe Seyler. 1988 Jul;369(7):567–571. doi: 10.1515/bchm3.1988.369.2.567. [DOI] [PubMed] [Google Scholar]
  7. Müller R., Thiele J., Klages U., Lingens F. Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from pseudomonas spec. CBS 3. Biochem Biophys Res Commun. 1984 Oct 15;124(1):178–182. doi: 10.1016/0006-291x(84)90933-1. [DOI] [PubMed] [Google Scholar]
  8. Ruisinger S., Klages U., Lingens F. Abbau der 4-chlorbenzoesäure durch eine Arthrobacter-Species. Arch Microbiol. 1976 Nov 2;110(23):253–256. doi: 10.1007/BF00690235. [DOI] [PubMed] [Google Scholar]
  9. van den Tweel W. J., Kok J. B., de Bont J. A. Reductive dechlorination of 2,4-dichlorobenzoate to 4-chlorobenzoate and hydrolytic dehalogenation of 4-chloro-, 4-bromo-, and 4-iodobenzoate by Alcaligenes denitrificans NTB-1. Appl Environ Microbiol. 1987 Apr;53(4):810–815. doi: 10.1128/aem.53.4.810-815.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES