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. 1991 Feb;57(2):499–502. doi: 10.1128/aem.57.2.499-502.1991

Characteristics of the adhesive determinants of Lactobacillus fermentum 104.

A Henriksson 1, R Szewzyk 1, P L Conway 1
PMCID: PMC182739  PMID: 1849714

Abstract

The adhesion of Lactobacillus fermentum 104-R and the variant strain 104-S to porcine gastric squamous epithelium was investigated. An epithelium-specific adhesion was detected for strain 104-S; however, strain 104-R expressed enhanced adhesion capacity to the control surfaces of polystyrene and bovine serum albumin. To characterize the adhesive determinants, the bacterial cells were exposed to various treatments. The adhesion pattern of bacterial cells in buffers of pH values ranging from 2 to 7 was determined. The adhesion of strain 104-S to epithelium was greater in a buffer with a higher pH value. On the other hand, adhesion of strain 104-R to the epithelium was rather unaffected by a change in pH. To the control surfaces of polystyrene or bovine serum albumin, the adhesion of both strains was greatest at pH 2 to 4. Treatment of strain 104-S with metaperiodate did not affect the adhesion to epithelium or polystyrene; however, protease treatment dramatically decreased the adhesion of both strains, thus suggesting that the determinants responsible for the adhesion were proteinaceous. Carbohydrates may be partially involved in the adhesion of 104-R because metaperiodate-treated cells adhered more poorly than control, iodate-treated cells. The adhesion-promoting components are most probably tightly bound to the cell wall, because washing with low-pH buffer (pH 1.2) or sodium dodecyl sulfate had no major effect on the adhesion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brooker B. E., Fuller R. Adhesion of Lactobacilli to the chicken crop epithelium. J Ultrastruct Res. 1975 Jul;52(1):21–31. doi: 10.1016/s0022-5320(75)80019-0. [DOI] [PubMed] [Google Scholar]
  2. Conway P. L., Adams R. F. Role of erythrosine in the inhibition of adhesion of Lactobacillus fermentum strain 737 to mouse stomach tissue. J Gen Microbiol. 1989 May;135(5):1167–1173. doi: 10.1099/00221287-135-5-1167. [DOI] [PubMed] [Google Scholar]
  3. Conway P. L., Kjelleberg S. Protein-mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. J Gen Microbiol. 1989 May;135(5):1175–1186. doi: 10.1099/00221287-135-5-1175. [DOI] [PubMed] [Google Scholar]
  4. Fuller R. Nature of the determinant responsible for the adhesion of lactobacilli to chicken crop epithelial cells. J Gen Microbiol. 1975 Apr;87(2):245–250. doi: 10.1099/00221287-87-2-245. [DOI] [PubMed] [Google Scholar]
  5. Hogg S. D., Manning J. E. Inhibition of adhesion of viridans streptococci to fibronectin-coated hydroxyapatite beads by lipoteichoic acid. J Appl Bacteriol. 1988 Dec;65(6):483–489. doi: 10.1111/j.1365-2672.1988.tb01921.x. [DOI] [PubMed] [Google Scholar]
  6. Husmark U., Rönner U. Forces involved in adhesion of Bacillus cereus spores to solid surfaces under different environmental conditions. J Appl Bacteriol. 1990 Oct;69(4):557–562. doi: 10.1111/j.1365-2672.1990.tb01548.x. [DOI] [PubMed] [Google Scholar]
  7. SMITH H. W. OBSERVATIONS ON THE FLORA OF THE ALIMENTARY TRACT OF ANIMALS AND FACTORS AFFECTING ITS COMPOSITION. J Pathol Bacteriol. 1965 Jan;89:95–122. [PubMed] [Google Scholar]
  8. Savage D. C. Associations and physiological interactions of indigenous microorganisms and gastrointestinal epithelia. Am J Clin Nutr. 1972 Dec;25(12):1372–1379. doi: 10.1093/ajcn/25.12.1372. [DOI] [PubMed] [Google Scholar]
  9. Sherman L. A., Savage D. C. Lipoteichoic acids in Lactobacillus strains that colonize the mouse gastric epithelium. Appl Environ Microbiol. 1986 Aug;52(2):302–304. doi: 10.1128/aem.52.2.302-304.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith H. W., Linggood M. A. Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. J Med Microbiol. 1971 Nov;4(4):467–485. doi: 10.1099/00222615-4-4-467. [DOI] [PubMed] [Google Scholar]
  11. Suegara N., Morotomi M., Watanabe T., Kawal Y., Mutai M. Behavior of microflora in the rat stomach: adhesion of lactobacilli to the keratinized epithelial cells of the rat stomach in vitro. Infect Immun. 1975 Jul;12(1):173–179. doi: 10.1128/iai.12.1.173-179.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wadström T., Andersson K., Sydow M., Axelsson L., Lindgren S., Gullmar B. Surface properties of lactobacilli isolated from the small intestine of pigs. J Appl Bacteriol. 1987 Jun;62(6):513–520. doi: 10.1111/j.1365-2672.1987.tb02683.x. [DOI] [PubMed] [Google Scholar]

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