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. 1991 Feb;57(2):586–591. doi: 10.1128/aem.57.2.586-591.1991

Detection of Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe.

P I Peterkin 1, E S Idziak 1, A N Sharpe 1
PMCID: PMC182753  PMID: 1901711

Abstract

A DNA probe specific for Listeria monocytogenes was isolated from a beta-hemolytic recombinant clone of an L. monocytogenes gene bank. It was labeled with horseradish peroxidase and used in a direct colony hybridization method on hydrophobic grid-membrane filters for the detection of the organism. Following color development of the chromogen, a commercial counter (HGMF Interpreter) was able to detect and count the organisms electronically. The method gave a positive reaction with 70 L. monocytogenes strains, while showing a negative reaction with 10 strains of other Listeria spp. and with 20 organisms of other genera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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