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. 1991 Feb;57(2):606–609. doi: 10.1128/aem.57.2.606-609.1991

Species-specific detection of Listeria monocytogenes by DNA amplification.

H G Deneer 1, I Boychuk 1
PMCID: PMC182759  PMID: 1901713

Abstract

The polymerase chain reaction was used to detect and specifically identify Listeria monocytogenes. A 174-bp region of the listeriolysin O gene was shown to be specifically amplified in L. monocytogenes but not in other species of Listeria or in a number of other gram-positive and gram-negative organisms. Less than 50 organisms could routinely be detected by a procedure involving two rounds of 35 amplification cycles each and without the need for subsequent hybridization with labeled probes.

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Selected References

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