Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Mar;57(3):843–846. doi: 10.1128/aem.57.3.843-846.1991

Portable Device for Preparation and Delivery of Gas Mixtures

I J Misaghi 1,*, L J Stowell 1
PMCID: PMC182804  PMID: 16348449

Abstract

A simple, portable device for the preparation and delivery of gas mixtures has been designed and constructed. The basic feature of the device is the use of gas flow controllers to maintain stable flow rates over a wide range of downstream pressures, instead of the capillary tubes and water-filled barostats commonly used in gas-mixing devices. Elimination of the barostat avoids problems such as water leakage, the loss of gases through the barostat, and changes in gas pressure due to evaporative loss of water from the barostat. The absence of a barostat also provides a closed system, allowing the use of the device for mixing and delivering of toxic gases. The prototype of the device has been used to prepare mixtures of different gases for more than 1 year and has been found to operate consistently and reproducibly. The actual concentrations of O2, CO2, and N2 in gas mixtures (determined by gas chromatography) immediately after mixing were between 2.2 and 6.6% of the desired values in four performance tests. Fluctuations in concentration of gases in mixtures after 9 days of continuous gas delivery was less than 2% in four performance tests.

Full text

PDF
843

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Enfors S. O., Molin G. Effect of high concentrations of carbon dioxide on growth rate of Pseudomonas fragi, Bacillus cereus and Streptococcus cremoris. J Appl Bacteriol. 1980 Jun;48(3):409–416. doi: 10.1111/j.1365-2672.1980.tb01029.x. [DOI] [PubMed] [Google Scholar]
  2. Gill C. O., Tan K. H. Effect of carbon dioxide on growth of Pseudomonas fluorescens. Appl Environ Microbiol. 1979 Aug;38(2):237–240. doi: 10.1128/aem.38.2.237-240.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES