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. 1991 Apr;57(4):949–954. doi: 10.1128/aem.57.4.949-954.1991

The Fe2+ Chelator Proferrorosamine A Is Essential for the Siderophore-Mediated Uptake of Iron by Pseudomonas roseus fluorescens

Marleen Vande Woestyne 1, Bart Bruyneel 1, Max Mergeay 1, Willy Verstraete 1,*
PMCID: PMC182828  PMID: 16348473

Abstract

Pseudomonas roseus fluorescens produces, besides the Fe2+ chelator proferrorosamine A, Fe3+ -chelating compounds, called siderophores. The production of proferrorosamine A and siderophores by P. roseus fluorescens appears to be controlled in a similar way by the concentration of available iron and by the concentration of dissolved oxygen. The higher the concentration of iron available for the microorganism, the lower the production of both chelating compounds. However, the production of siderophores was much more sensitive to iron availability than was proferrorosamine A production. Proferrorosamine A and siderophores were only produced in minimal medium C if the concentration of dissolved oxygen ranged from 4.5 to 2.0 ppm. At higher or lower concentrations, none of the iron-chelating compounds were produced. Furthermore, it has been shown that proferrorosamine-negative Tn5 mutants of P. roseus fluorescens were able to form siderophores only under iron-limiting conditions when proferrorosamine A was added to the medium. Our data suggest that proferrorosamine A production is essential for siderophore synthesis by P. roseus fluorescens; the production of siderophores occurred only when proferrorosamine A was present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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