Genomic DNA functions as a universal external standard in quantitative real-time PCR
J. J. Yun, L. E. Heisler, I. I. Hwang, O. Wilkins, S. K. Lau, M. Hyrcza, B. Jayabalasingham, J. Jin, J. McLaurin, M. S. Tsao and S. D. Der
The authors apologize for an error in Materials and Methods in the above paper.
The final concentration of primers in a single real-time PCR should have been written as 0.5 μM (micro) not 1 nM (nano).
The full and correct sentence is given below.
Each 10 ml reaction contained 1× PCR buffer (Sigma–Aldrich Co.), 3 mM MgCl2, 0.2 mM dNTP, 0.5 μM forward and reverse primers, 1:50 dilution of ROX reference dye (Sigma–Aldrich Co.), 3:100 000 dilution of SYBR Green I (Sigma–Aldrich Co.), 0.05 U of JumpStart Taq polymerase (Sigma–Aldrich Co.) and template DNA.