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. 1991 Apr;57(4):1202–1206. doi: 10.1128/aem.57.4.1202-1206.1991

Growth and survival of Bordetella bronchiseptica in natural waters and in buffered saline without added nutrients.

J F Porter 1, R Parton 1, A C Wardlaw 1
PMCID: PMC182868  PMID: 2059041

Abstract

Bordetella bronchiseptica showed increases in viable count when incubated in phosphate-buffered saline (PBS), in reagent-grade water, and in local lake and pond waters, all without added nutrients. Within 48 to 72 h at 37 degrees C in PBS and in lake and pond waters, stationary-phase populations of around 2.7 x 10(6) CFU/ml developed from washed B. bronchiseptica inocula of around 2 x 10(3) CFU/ml. Increases in CFU on the order of five- and eightfold, respectively, were observed in reagent-grade water and in seawater from the same sizes of inocula. The organisms remained viable for at least 3 weeks in PBS and in lake waters at 37 degrees C. The possibility that carry-over of nutrients was responsible for growth was discounted by showing serial transfer of B. bronchiseptica in PBS under conditions in which Escherichia coli tested in parallel rapidly died out.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ELDERING G., EVELAND W. C., KENDRICK P. L. Fluorescent antibody staining and agglutination reactions in Bordetella pertussis cultures. J Bacteriol. 1962 Apr;83:745–749. doi: 10.1128/jb.83.4.745-749.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GARVIE E. I. The growth of Escherichia coli in buffer substrate and distilled water. J Bacteriol. 1955 Apr;69(4):393–398. doi: 10.1128/jb.69.4.393-398.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goodnow R. A. Biology of Bordetella bronchiseptica. Microbiol Rev. 1980 Dec;44(4):722–738. doi: 10.1128/mr.44.4.722-738.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Harder W., Dijkhuizen L. Physiological responses to nutrient limitation. Annu Rev Microbiol. 1983;37:1–23. doi: 10.1146/annurev.mi.37.100183.000245. [DOI] [PubMed] [Google Scholar]
  5. Imaizumi A., Suzuki Y., Ono S., Sato H., Sato Y. Effect of heptakis (2,6-O-dimethyl) beta-cyclodextrin on the production of pertussis toxin by Bordetella pertussis. Infect Immun. 1983 Sep;41(3):1138–1143. doi: 10.1128/iai.41.3.1138-1143.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Novitsky J. A., Morita R. Y. Survival of a psychrophilic marine Vibrio under long-term nutrient starvation. Appl Environ Microbiol. 1977 Mar;33(3):635–641. doi: 10.1128/aem.33.3.635-641.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. POSTGATE J. R., HUNTER J. R. The survival of starved bacteria. J Gen Microbiol. 1962 Oct;29:233–263. doi: 10.1099/00221287-29-2-233. [DOI] [PubMed] [Google Scholar]
  8. PROOM H. The minimal nutritional requirements of organisms of the genus Bordetella López. J Gen Microbiol. 1955 Feb;12(1):63–75. doi: 10.1099/00221287-12-1-63. [DOI] [PubMed] [Google Scholar]
  9. SWITZER W. P. Studies on infectious atrophic rhinitis. V. Concept that several agents may cause turbinate atrophy. Am J Vet Res. 1956 Jul;17(64):478–484. [PubMed] [Google Scholar]
  10. Thompson H., McCandlish I. A., Wright N. G. Experimental respiratory disease in dogs due to Bordetella bronchiseptica. Res Vet Sci. 1976 Jan;20(1):16–23. [PubMed] [Google Scholar]

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