Abstract
Addition of copper to the medium for Methylomonas albus BG8 increased cell yield and methane monooxygenase activity. Intracytoplasmic membrane was formed only in cells grown with copper supplementation. Additionally, the abundances of two major membrane proteins were affected by copper in the growth medium. These findings indicate that effects of copper on the physiology of methanotrophic bacteria are not limited to those on types II and X.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Crook S. M., Treml S. B., Collins M. L. Immunocytochemical ultrastructural analysis of chromatophore membrane formation in Rhodospirillum rubrum. J Bacteriol. 1986 Jul;167(1):89–95. doi: 10.1128/jb.167.1.89-95.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fassel T. A., Schaller M. J., Lidstrom M. E., Remsen C. C. Effect of fixation-resin combinations and ruthenium red on elucidating outer envelope structure and surface morphology of two methanotrophic bacteria. J Electron Microsc Tech. 1990 Jan;14(1):52–62. doi: 10.1002/jemt.1060140109. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Mueller P. R., Collins M. L. Identification of two distinct lactate dehydrogenases in Rhodospirillum rubrum. J Bacteriol. 1983 Mar;153(3):1562–1566. doi: 10.1128/jb.153.3.1562-1566.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myers C. R., Collins M. L. Cell-cycle-specific oscillation in the composition of chromatophore membrane in Rhodospirillum rubrum. J Bacteriol. 1986 Jun;166(3):818–823. doi: 10.1128/jb.166.3.818-823.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takeda K., Tanaka K. Ultrastructure of intracytoplasmic membranes of Methanomonas margaritae cells grown under different conditions. Antonie Van Leeuwenhoek. 1980;46(1):15–25. doi: 10.1007/BF00422225. [DOI] [PubMed] [Google Scholar]
- Whittenbury R., Phillips K. C., Wilkinson J. F. Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol. 1970 May;61(2):205–218. doi: 10.1099/00221287-61-2-205. [DOI] [PubMed] [Google Scholar]