Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 May;57(5):1535–1539. doi: 10.1128/aem.57.5.1535-1539.1991

Total coliform detection in drinking water: comparison of membrane filtration with Colilert and Coliquik.

B H Olson 1, D L Clark 1, B B Milner 1, M H Stewart 1, R L Wolfe 1
PMCID: PMC182981  PMID: 1854206

Abstract

The Colilert (CL) and Coliquik (CQ) systems were compared in a presence-absence format against the Standard Methods membrane filtration (MF) technique to determine whether differences existed in total coliform detection. Approximately 750 water samples were collected from distribution systems, covered and uncovered storage reservoirs, well sites, and the influent to drinking water treatment plants. Samples were analyzed for total coliforms and heterotrophic bacteria with MF, CL, and CQ. The agreements between CL and MF and between CQ and MF were both greater than 94.8%, which indicates that both may be acceptable methods for total coliform detection. Disagreement between the CL and CQ methods was primarily due to false-negative results. Furthermore, laboratory and field inoculation methods were compared for CL, more than 98% agreement was obtained. This finding indicates that sampling and immediate field inoculation may be an alternative to the traditional laboratory inoculation.

Full text

PDF
1535

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang G. W., Brill J., Lum R. Proportion of beta-D-glucuronidase-negative Escherichia coli in human fecal samples. Appl Environ Microbiol. 1989 Feb;55(2):335–339. doi: 10.1128/aem.55.2.335-339.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark D. L., Milner B. B., Stewart M. H., Wolfe R. L., Olson B. H. Comparative study of commercial 4-methylumbelliferyl-beta-D-glucuronide preparations with the Standard Methods membrane filtration fecal coliform test for the detection of Escherichia coli in water samples. Appl Environ Microbiol. 1991 May;57(5):1528–1534. doi: 10.1128/aem.57.5.1528-1534.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Covert T. C., Shadix L. C., Rice E. W., Haines J. R., Freyberg R. W. Evaluation of the Autoanalysis Colilert test for detection and enumeration of total coliforms. Appl Environ Microbiol. 1989 Oct;55(10):2443–2447. doi: 10.1128/aem.55.10.2443-2447.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis W. A., 2nd, Kane J. G., Garagusi V. F. Human aeromonas infections: a review of the literature and a case report of endocarditis. Medicine (Baltimore) 1978 May;57(3):267–277. [PubMed] [Google Scholar]
  5. Edberg S. C., Allen M. J., Smith D. B., Kriz N. J. Enumeration of total coliforms and Escherichia coli from source water by the defined substrate technology. Appl Environ Microbiol. 1990 Feb;56(2):366–369. doi: 10.1128/aem.56.2.366-369.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edberg S. C., Allen M. J., Smith D. B. National field evaluation of a defined substrate method for the simultaneous enumeration of total coliforms and Escherichia coli from drinking water: comparison with the standard multiple tube fermentation method. Appl Environ Microbiol. 1988 Jun;54(6):1595–1601. doi: 10.1128/aem.54.6.1595-1601.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edberg S. C., Edberg M. M. A defined substrate technology for the enumeration of microbial indicators of environmental pollution. Yale J Biol Med. 1988 Sep-Oct;61(5):389–399. [PMC free article] [PubMed] [Google Scholar]
  8. Feng P., Lum R., Chang G. W. Identification of uidA gene sequences in beta-D-glucuronidase-negative Escherichia coli. Appl Environ Microbiol. 1991 Jan;57(1):320–323. doi: 10.1128/aem.57.1.320-323.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ketover B. P., Young L. S., Armstrong D. Septicemia due to Aeromonas hydrophila: clinical and immunologic aspects. J Infect Dis. 1973 Mar;127(3):284–290. doi: 10.1093/infdis/127.3.284. [DOI] [PubMed] [Google Scholar]
  10. Lewis C. M., Mak J. L. Comparison of membrane filtration and Autoanalysis Colilert presence-absence techniques for analysis of total coliforms and Escherichia coli in drinking water samples. Appl Environ Microbiol. 1989 Dec;55(12):3091–3094. doi: 10.1128/aem.55.12.3091-3094.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reasoner D. J., Geldreich E. E. A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol. 1985 Jan;49(1):1–7. doi: 10.1128/aem.49.1.1-7.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES