Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 May;57(5):1573–1575. doi: 10.1128/aem.57.5.1573-1575.1991

Immunofluorescence and morphology of Giardia lamblia cysts exposed to chlorine.

J F Sauch 1, D Berman 1
PMCID: PMC182989  PMID: 1854208

Abstract

Giardia cyst-like objects detected by immunofluorescence in chlorinated water samples often cannot be positively identified by their morphological appearance. To determine the effect of chlorine on cyst immunofluorescence and morphology, Giardia lamblia cysts were exposed to chlorine for 48 h. The majority of cysts exposed to chlorine concentrations of 1 to 11 mg/liter at 5 and 15 degrees C lost their internal morphological characteristics necessary for identification, but most of them were still detectable by immunofluorescence.

Full text

PDF
1573

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belosevic M., Faubert G. M., MacLean J. D., Law C., Croll N. A. Giardia lamblia infections in Mongolian gerbils: an animal model. J Infect Dis. 1983 Feb;147(2):222–226. doi: 10.1093/infdis/147.2.222. [DOI] [PubMed] [Google Scholar]
  2. Roberts-Thomson I. C., Stevens D. P., Mahmoud A. A., Warren K. S. Giardiasis in the mouse: an animal model. Gastroenterology. 1976 Jul;71(1):57–61. [PubMed] [Google Scholar]
  3. Rose J. B., Landeen L. K., Riley K. R., Gerba C. P. Evaluation of immunofluorescence techniques for detection of Cryptosporidium oocysts and Giardia cysts from environmental samples. Appl Environ Microbiol. 1989 Dec;55(12):3189–3196. doi: 10.1128/aem.55.12.3189-3196.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Sauch J. F. Purification of Giardia muris cysts by velocity sedimentation. Appl Environ Microbiol. 1984 Aug;48(2):454–455. doi: 10.1128/aem.48.2.454-455.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sauch J. F. Use of immunofluorescence and phase-contrast microscopy for detection and identification of Giardia cysts in water samples. Appl Environ Microbiol. 1985 Dec;50(6):1434–1438. doi: 10.1128/aem.50.6.1434-1438.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES