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. 1992 Sep;58(9):3095–3100. doi: 10.1128/aem.58.9.3095-3100.1992

Detection of virulence factors in culturable Escherichia coli isolates from water samples by DNA probes and recovery of toxin-bearing strains in minimal o-nitrophenol-beta-D-galactopyranoside-4-methylumbelliferyl-beta-D-g luc uronide media.

M T Martins 1, I G Rivera 1, D L Clark 1, B H Olson 1
PMCID: PMC183054  PMID: 1444424

Abstract

A total of 449 Escherichia coli isolates in treated and raw water sources were submitted to DNA-DNA hybridization using seven different DNA probes to detect homology to sequences that code for Shiga-like toxins I and II; heat-stabile and heat-labile toxins, adherence factors EAF and eae, and the fimbrial antigen of entero-hemorrhagic E. coli. Fifty-nine (13%) of the isolates demonstrated homology with one or more specific DNA probes. More than 50% of the isolates in treated water were not recovered in MMO-4-methylumbelliferyl-beta-D-glucuronide media designed for detection of this indicator.

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Selected References

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  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bohnert M. G., d'Hauteville H. M., Sansonetti P. J. Detection of enteric pathotypes of Escherichia coli by hybridization using six DNA probes. Ann Inst Pasteur Microbiol. 1988 Mar-Apr;139(2):189–202. [PubMed] [Google Scholar]
  3. Brown J. E., Echeverria P., Taylor D. N., Seriwatana J., Vanapruks V., Lexomboon U., Neill R. N., Newland J. W. Determination by DNA hybridization of Shiga-like-toxin-producing Escherichia coli in children with diarrhea in Thailand. J Clin Microbiol. 1989 Feb;27(2):291–294. doi: 10.1128/jcm.27.2.291-294.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark D. L., Milner B. B., Stewart M. H., Wolfe R. L., Olson B. H. Comparative study of commercial 4-methylumbelliferyl-beta-D-glucuronide preparations with the Standard Methods membrane filtration fecal coliform test for the detection of Escherichia coli in water samples. Appl Environ Microbiol. 1991 May;57(5):1528–1534. doi: 10.1128/aem.57.5.1528-1534.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dallas W. S., Gill D. M., Falkow S. Cistrons encoding Escherichia coli heat-labile toxin. J Bacteriol. 1979 Sep;139(3):850–858. doi: 10.1128/jb.139.3.850-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Echeverria P., Seriwatana J., Chityothin O., Chaicumpa W., Tirapat C. Detection of enterotoxigenic Escherichia coli in water by filter hybridization with three enterotoxin gene probes. J Clin Microbiol. 1982 Dec;16(6):1086–1090. doi: 10.1128/jcm.16.6.1086-1090.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Echeverria P., Seriwatana J., Leksomboon U., Tirapat C., Chaicumpa W., Rowe B. Identification by DNA hybridisation of enterotoxigenic Escherichia coli in homes of children with diarrhoea. Lancet. 1984 Jan 14;1(8368):63–66. doi: 10.1016/s0140-6736(84)90001-1. [DOI] [PubMed] [Google Scholar]
  8. Echeverria P., Seriwatana J., Patamaroj U., Moseley S. L., McFarland A., Chityothin O., Chaicumpa W. Prevalence of heat-stable II enterotoxigenic Escherichia coli in pigs, water, and people at farms in Thailand as determined by DNA hybridization. J Clin Microbiol. 1984 Apr;19(4):489–491. doi: 10.1128/jcm.19.4.489-491.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Echeverria P., Seriwatana J., Taylor D. N., Tirapat C., Chaicumpa W., Rowe B. Identification by DNA hybridization of enterotoxigenic Escherichia coli in a longitudinal study of villages in Thailand. J Infect Dis. 1985 Jan;151(1):124–130. doi: 10.1093/infdis/151.1.124. [DOI] [PubMed] [Google Scholar]
  10. Gomes T. A., Toledo M. R., Trabulsi L. R., Wood P. K., Morris J. G., Jr DNA probes for identification of enteroinvasive Escherichia coli. J Clin Microbiol. 1987 Oct;25(10):2025–2027. doi: 10.1128/jcm.25.10.2025-2027.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jefferson R. A., Burgess S. M., Hirsh D. beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447–8451. doi: 10.1073/pnas.83.22.8447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jerse A. E., Martin W. C., Galen J. E., Kaper J. B. Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC. J Clin Microbiol. 1990 Dec;28(12):2842–2844. doi: 10.1128/jcm.28.12.2842-2844.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jerse A. E., Yu J., Tall B. D., Kaper J. B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839–7843. doi: 10.1073/pnas.87.20.7839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Krishnan C., Fitzgerald V. A., Dakin S. J., Behme R. J. Laboratory investigation of outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7. J Clin Microbiol. 1987 Jun;25(6):1043–1047. doi: 10.1128/jcm.25.6.1043-1047.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Levine M. M. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis. 1987 Mar;155(3):377–389. doi: 10.1093/infdis/155.3.377. [DOI] [PubMed] [Google Scholar]
  16. Levine M. M., Xu J. G., Kaper J. B., Lior H., Prado V., Tall B., Nataro J., Karch H., Wachsmuth K. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis. 1987 Jul;156(1):175–182. doi: 10.1093/infdis/156.1.175. [DOI] [PubMed] [Google Scholar]
  17. McGowan K. L., Wickersham E., Strockbine N. A. Escherichia coli O157:H7 from water. Lancet. 1989 Apr 29;1(8644):967–968. doi: 10.1016/s0140-6736(89)92559-2. [DOI] [PubMed] [Google Scholar]
  18. Montenegro M. A., Bülte M., Trumpf T., Aleksić S., Reuter G., Bulling E., Helmuth R. Detection and characterization of fecal verotoxin-producing Escherichia coli from healthy cattle. J Clin Microbiol. 1990 Jun;28(6):1417–1421. doi: 10.1128/jcm.28.6.1417-1421.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moseley S. L., Echeverria P., Seriwatana J., Tirapat C., Chaicumpa W., Sakuldaipeara T., Falkow S. Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes. J Infect Dis. 1982 Jun;145(6):863–869. doi: 10.1093/infdis/145.6.863. [DOI] [PubMed] [Google Scholar]
  20. Moseley S. L., Hardy J. W., Hug M. I., Echeverria P., Falkow S. Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli. Infect Immun. 1983 Mar;39(3):1167–1174. doi: 10.1128/iai.39.3.1167-1174.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Moseley S. L., Huq I., Alim A. R., So M., Samadpour-Motalebi M., Falkow S. Detection of enterotoxigenic Escherichia coli by DNA colony hybridization. J Infect Dis. 1980 Dec;142(6):892–898. doi: 10.1093/infdis/142.6.892. [DOI] [PubMed] [Google Scholar]
  22. Nataro J. P., Baldini M. M., Kaper J. B., Black R. E., Bravo N., Levine M. M. Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. J Infect Dis. 1985 Sep;152(3):560–565. doi: 10.1093/infdis/152.3.560. [DOI] [PubMed] [Google Scholar]
  23. Newland J. W., Neill R. J. DNA probes for Shiga-like toxins I and II and for toxin-converting bacteriophages. J Clin Microbiol. 1988 Jul;26(7):1292–1297. doi: 10.1128/jcm.26.7.1292-1297.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Newland J. W., Strockbine N. A., Neill R. J. Cloning of genes for production of Escherichia coli Shiga-like toxin type II. Infect Immun. 1987 Nov;55(11):2675–2680. doi: 10.1128/iai.55.11.2675-2680.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Olson B. H., Clark D. L., Milner B. B., Stewart M. H., Wolfe R. L. Total coliform detection in drinking water: comparison of membrane filtration with Colilert and Coliquik. Appl Environ Microbiol. 1991 May;57(5):1535–1539. doi: 10.1128/aem.57.5.1535-1539.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Raghubeer E. V., Matches J. R. Temperature range for growth of Escherichia coli serotype O157:H7 and selected coliforms in E. coli medium. J Clin Microbiol. 1990 Apr;28(4):803–805. doi: 10.1128/jcm.28.4.803-805.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Samadpour M., Liston J., Ongerth J. E., Tarr P. I. Evaluation of DNA probes for detection of Shiga-like-toxin-producing Escherichia coli in food and calf fecal samples. Appl Environ Microbiol. 1990 May;56(5):1212–1215. doi: 10.1128/aem.56.5.1212-1215.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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