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. 1992 Sep;58(9):3177–3179. doi: 10.1128/aem.58.9.3177-3179.1992

Evaluation of the ability of primary selective enrichment to resuscitate heat-injured and freeze-injured Listeria monocytogenes cells.

E Budu-Amoako 1, S Toora 1, R F Ablett 1, J Smith 1
PMCID: PMC183068  PMID: 1444432

Abstract

Resuscitation rates of injured Listeria monocytogenes on conventional selective Listeria enrichment broth and nonselective Trypticase soy broth containing 0.6% yeast extract were compared. Cells were heated to 60 degrees C for 5 min or frozen at -20 degrees C for 7 days. Inoculation of Trypticase soy broth-yeast extract with the stressed cells resulted in growth that was superior to that in Listeria enrichment broth. Injured cells were fully recovered at 6 to 8 h.

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Selected References

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  1. Buchanan R. L., Stahl H. G., Bencivengo M. M., Del Corral F. Comparison of lithium chloride-phenylethanol-moxalactam and modified Vogel Johnson agars for detection of Listeria spp. in retail-level meats, poultry, and seafood. Appl Environ Microbiol. 1989 Mar;55(3):599–603. doi: 10.1128/aem.55.3.599-603.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Busch S. V., Donnelly C. W. Development of a repair-enrichment broth for resuscitation of heat-injured Listeria monocytogenes and Listeria innocua. Appl Environ Microbiol. 1992 Jan;58(1):14–20. doi: 10.1128/aem.58.1.14-20.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins-Thompson D. L., Hurst A., Kruse H. Synthesis of enterotoxin B by Staphylococcus aureus strain S6 after recovery from heat injury. Can J Microbiol. 1973 Dec;19(12):1463–1468. doi: 10.1139/m73-238. [DOI] [PubMed] [Google Scholar]
  4. Doyle M. P., Schoeni J. L. Selective-enrichment procedure for isolation of Listeria monocytogenes from fecal and biologic specimens. Appl Environ Microbiol. 1986 May;51(5):1127–1129. doi: 10.1128/aem.51.5.1127-1129.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  6. Golden D. A., Beuchat L. R., Brackett R. E. Evaluation of selective direct plating media for their suitability to recover uninjured, heat-injured, and freeze-injured Listeria monocytogenes from foods. Appl Environ Microbiol. 1988 Jun;54(6):1451–1456. doi: 10.1128/aem.54.6.1451-1456.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hao D. Y., Beuchat L. R., Brackett R. E. Comparison of media and methods for detecting and enumerating Listeria monocytogenes in refrigerated cabbage. Appl Environ Microbiol. 1987 May;53(5):955–957. doi: 10.1128/aem.53.5.955-957.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Linnan M. J., Mascola L., Lou X. D., Goulet V., May S., Salminen C., Hird D. W., Yonekura M. L., Hayes P., Weaver R. Epidemic listeriosis associated with Mexican-style cheese. N Engl J Med. 1988 Sep 29;319(13):823–828. doi: 10.1056/NEJM198809293191303. [DOI] [PubMed] [Google Scholar]
  9. Sorrells K. M., Speck M. L., Warren J. A. Pathogenicity of Salmonella gallinarum after metabolic injury by freezing. Appl Microbiol. 1970 Jan;19(1):39–43. doi: 10.1128/am.19.1.39-43.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Speck M. L., Ray B., Read R. B., Jr Repair and enumeration of injured coliforms by a plating procedure. Appl Microbiol. 1975 Apr;29(4):549–550. doi: 10.1128/am.29.4.549-550.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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