Abstract
Two dead-end metabolites of anaerobic toluene transformation, benzylsuccinic acid and benzylfumaric acid, accumulated in sulfate-reducing enrichment cultures that were fed toluene as the sole carbon source. Stable isotope-labeled toluene and gas chromatography-mass spectrometry were used to confirm that the compounds resulted from toluene metabolism. The two metabolites constituted less than 10% of the toluene carbon (over 80% was mineralized to carbon dioxide, according to a previous study). This study demonstrates that the novel nonproductive pathway proposed by Evans and coworkers (P. J. Evans, W. Ling, B. Goldschmidt, E. R. Ritter, and L. Y. Young, Appl. Environ. Microbiol. 58:496-501, 1992) for a denitrifying pure culture applies to disparate anaerobic bacteria.
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Selected References
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- Altenschmidt U., Fuchs G. Anaerobic degradation of toluene in denitrifying Pseudomonas sp.: indication for toluene methylhydroxylation and benzoyl-CoA as central aromatic intermediate. Arch Microbiol. 1991;156(2):152–158. doi: 10.1007/BF00290990. [DOI] [PubMed] [Google Scholar]
- Beller H. R., Grbić-Galić D., Reinhard M. Microbial degradation of toluene under sulfate-reducing conditions and the influence of iron on the process. Appl Environ Microbiol. 1992 Mar;58(3):786–793. doi: 10.1128/aem.58.3.786-793.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans P. J., Ling W., Goldschmidt B., Ritter E. R., Young L. Y. Metabolites formed during anaerobic transformation of toluene and o-xylene and their proposed relationship to the initial steps of toluene mineralization. Appl Environ Microbiol. 1992 Feb;58(2):496–501. doi: 10.1128/aem.58.2.496-501.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grbić-Galić D., Vogel T. M. Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol. 1987 Feb;53(2):254–260. doi: 10.1128/aem.53.2.254-260.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuhn E. P., Zeyer J., Eicher P., Schwarzenbach R. P. Anaerobic degradation of alkylated benzenes in denitrifying laboratory aquifer columns. Appl Environ Microbiol. 1988 Feb;54(2):490–496. doi: 10.1128/aem.54.2.490-496.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lovley D. R., Lonergan D. J. Anaerobic Oxidation of Toluene, Phenol, and p-Cresol by the Dissimilatory Iron-Reducing Organism, GS-15. Appl Environ Microbiol. 1990 Jun;56(6):1858–1864. doi: 10.1128/aem.56.6.1858-1864.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schocher R. J., Seyfried B., Vazquez F., Zeyer J. Anaerobic degradation of toluene by pure cultures of denitrifying bacteria. Arch Microbiol. 1991;157(1):7–12. doi: 10.1007/BF00245327. [DOI] [PubMed] [Google Scholar]
- Vogel T. M., Grbìc-Galìc D. Incorporation of Oxygen from Water into Toluene and Benzene during Anaerobic Fermentative Transformation. Appl Environ Microbiol. 1986 Jul;52(1):200–202. doi: 10.1128/aem.52.1.200-202.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]