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. 1992 Oct;58(10):3217–3224. doi: 10.1128/aem.58.10.3217-3224.1992

Solubilization and Mineralization of Lignin by White Rot Fungi

C David Boyle 1,†,*, Bradley R Kropp 1,‡, Ian D Reid 1,§
PMCID: PMC183083  PMID: 16348781

Abstract

The white rot fungi Lentinula edodes, Phanerochaete chrysosporium, Pleurotus sajor-caju, Flammulina velutipes, and Schizophyllum commune were grown in liquid media containing 14C-lignin-labelled wood, and the formation of water-soluble 14C-labelled products and 14CO2, the growth of the fungi, and the activities of extracellular lignin peroxidase, manganese peroxidase, and laccase were measured. Conditions that affect the rate of lignin degradation were imposed, and both long-term (0- to 16-day) and short-term (0- to 72-h) effects on the production of the two types of product and on the activities of the enzymes were monitored. The production of 14CO2-labelled products from the aqueous ones was also investigated. The short-term studies showed that the different conditions had different effects on the production of the two products and on the activities of the enzymes. Nitrogen sources inhibited the production of both products by all species when differences in growth could be discounted. Medium pH and manganese affected lignin degradation by the different species differently. With P. chrysosporium, the results were consistent, with lignin peroxidase playing a role in lignin solubilization and manganese peroxidase being important in subsequent CO2 production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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