Abstract
Addition of acetylene to microcosms simultaneously amended with nitrate and alkylbenzenes resulted in inhibition of the rate of alkylbenzene biodegradation under denitrifying conditions. Toluene, xylenes, and 1,2,4-trimethylbenzene were recalcitrant, whereas ethylbenzene was degraded at a slower rate than usual. Benzene was not degraded in either case. Addition of acetylene to microcosms preexposed to nitrate and alkylbenzenes produced similar inhibition. These data indicate that the activities of microorganisms that degrade alkylbenzenes under denitrifying conditions may be suppressed if the standard acetylene block technique is used to verify denitrifying activity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Altenschmidt U., Fuchs G. Anaerobic degradation of toluene in denitrifying Pseudomonas sp.: indication for toluene methylhydroxylation and benzoyl-CoA as central aromatic intermediate. Arch Microbiol. 1991;156(2):152–158. doi: 10.1007/BF00290990. [DOI] [PubMed] [Google Scholar]
- Dolfing J., Zeyer J., Binder-Eicher P., Schwarzenbach R. P. Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen. Arch Microbiol. 1990;154(4):336–341. doi: 10.1007/BF00276528. [DOI] [PubMed] [Google Scholar]
- Evans P. J., Mang D. T., Young L. Y. Degradation of toluene and m-xylene and transformation of o-xylene by denitrifying enrichment cultures. Appl Environ Microbiol. 1991 Feb;57(2):450–454. doi: 10.1128/aem.57.2.450-454.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchins S. R. Biodegradation of monoaromatic hydrocarbons by aquifer microorganisms using oxygen, nitrate, or nitrous oxide as the terminal electron acceptor. Appl Environ Microbiol. 1991 Aug;57(8):2403–2407. doi: 10.1128/aem.57.8.2403-2407.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuhn E. P., Zeyer J., Eicher P., Schwarzenbach R. P. Anaerobic degradation of alkylated benzenes in denitrifying laboratory aquifer columns. Appl Environ Microbiol. 1988 Feb;54(2):490–496. doi: 10.1128/aem.54.2.490-496.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schocher R. J., Seyfried B., Vazquez F., Zeyer J. Anaerobic degradation of toluene by pure cultures of denitrifying bacteria. Arch Microbiol. 1991;157(1):7–12. doi: 10.1007/BF00245327. [DOI] [PubMed] [Google Scholar]
- Topp E., Germon J. C. Acetylene metabolism and stimulation of denitrification in an agricultural soil. Appl Environ Microbiol. 1986 Oct;52(4):802–806. doi: 10.1128/aem.52.4.802-806.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeyer J., Kuhn E. P., Schwarzenbach R. P. Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl Environ Microbiol. 1986 Oct;52(4):944–947. doi: 10.1128/aem.52.4.944-947.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]