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. 1992 Nov;58(11):3593–3597. doi: 10.1128/aem.58.11.3593-3597.1992

Properties of a genetically reconstructed Prevotella ruminicola endoglucanase.

G Maglione 1, O Matsushita 1, J B Russell 1, D B Wilson 1
PMCID: PMC183149  PMID: 1482181

Abstract

A pUC19-derived plasmid was constructed that coded for a hybrid cellulase with the Thermomonospora fusca E2 cellulose-binding domain at its C terminus joined to the Prevotella ruminicola 40.5-kDa carboxymethyl cellulase (CMCase). The hybrid enzyme was purified and characterized enzymatically. It bound tightly to cellulose, and its specific activities on carboxymethyl cellulose, amorphous cellulose, and ball-milled cellulose were 1.5, 10, and 8 times that of the 40.5-kDa CMCase, respectively. Furthermore, the modified enzyme gave synergism with an exocellulase in the degradation of filter paper, while the 40.5-kDa CMCase did not.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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