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. 1992 Dec;58(12):3868–3872. doi: 10.1128/aem.58.12.3868-3872.1992

Monoclonal antibodies that detect live salmonellae.

R Torensma 1, M J Visser 1, C J Aarsman 1, M J Poppelier 1, R van Beurden 1, A C Fluit 1, J Verhoef 1
PMCID: PMC183196  PMID: 1476430

Abstract

Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were reactive with serogroups A, B, C1, C2, D, E, and K and Salmonella choleraesuis subsp. diarizonae. Each monoclonal antibody proved to be reactive with a distinct serotype. Clinical isolates belonging to these Salmonella serogroups could be detected. Reactivity with non-Salmonella bacteria proved to be minor.

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Selected References

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