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. 1992 Dec;58(12):3959–3963. doi: 10.1128/aem.58.12.3959-3963.1992

Effect of sodium chloride on the intracellular solute pools of Listeria monocytogenes.

R A Patchett 1, A F Kelly 1, R G Kroll 1
PMCID: PMC183211  PMID: 1476439

Abstract

The concentrations of intracellular solutes in Listeria monocytogenes were examined in cells grown at various concentrations of NaCl. At 5% NaCl, cells contained elevated concentrations of potassium and glycine betaine compared with concentrations in cells grown without NaCl. At 7.5% NaCl, cells contained increased concentrations of K+, glycine betaine, glycine, alanine, and proline. Only glycine betaine, choline, or glycine promoted growth on a solidified defined medium containing 4% NaCl; there was no growth at higher concentrations of NaCl in the defined medium.

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Selected References

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  1. Anderson C. B., Witter L. D. Glutamine and proline accumulation by Staphylococcus aureus with reduction in water activity. Appl Environ Microbiol. 1982 Jun;43(6):1501–1503. doi: 10.1128/aem.43.6.1501-1503.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cairney J., Booth I. R., Higgins C. F. Osmoregulation of gene expression in Salmonella typhimurium: proU encodes an osmotically induced betaine transport system. J Bacteriol. 1985 Dec;164(3):1224–1232. doi: 10.1128/jb.164.3.1224-1232.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cairney J., Booth I. R., Higgins C. F. Salmonella typhimurium proP gene encodes a transport system for the osmoprotectant betaine. J Bacteriol. 1985 Dec;164(3):1218–1223. doi: 10.1128/jb.164.3.1218-1223.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Csonka L. N. Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev. 1989 Mar;53(1):121–147. doi: 10.1128/mr.53.1.121-147.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gaillard J. L., Berche P., Sansonetti P. Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect Immun. 1986 Apr;52(1):50–55. doi: 10.1128/iai.52.1.50-55.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gray M. L., Killinger A. H. Listeria monocytogenes and listeric infections. Bacteriol Rev. 1966 Jun;30(2):309–382. doi: 10.1128/br.30.2.309-382.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang J. C., Huang H. S., Jurima-Romet M., Ashton F., Thomas B. H. Hepatocidal toxicity of Listeria species. FEMS Microbiol Lett. 1990 Nov;60(3):249–252. doi: 10.1016/0378-1097(90)90312-e. [DOI] [PubMed] [Google Scholar]
  8. Killham K., Firestone M. K. Salt stress control of intracellular solutes in streptomycetes indigenous to saline soils. Appl Environ Microbiol. 1984 Feb;47(2):301–306. doi: 10.1128/aem.47.2.301-306.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koch A. L. Shrinkage of growing Escherichia coli cells by osmotic challenge. J Bacteriol. 1984 Sep;159(3):919–924. doi: 10.1128/jb.159.3.919-924.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koujima I., Hayashi H., Tomochika K., Okabe A., Kanemasa Y. Adaptational change in proline and water content of Staphylococcus aureus after alteration of environmental salt concentration. Appl Environ Microbiol. 1978 Mar;35(3):467–470. doi: 10.1128/aem.35.3.467-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuhn M., Goebel W. Identification of an extracellular protein of Listeria monocytogenes possibly involved in intracellular uptake by mammalian cells. Infect Immun. 1989 Jan;57(1):55–61. doi: 10.1128/iai.57.1.55-61.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Landfald B., Strøm A. R. Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli. J Bacteriol. 1986 Mar;165(3):849–855. doi: 10.1128/jb.165.3.849-855.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Larsen P. I., Sydnes L. K., Landfald B., Strøm A. R. Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose. Arch Microbiol. 1987 Feb;147(1):1–7. doi: 10.1007/BF00492896. [DOI] [PubMed] [Google Scholar]
  14. Stock J. B., Rauch B., Roseman S. Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem. 1977 Nov 10;252(21):7850–7861. [PubMed] [Google Scholar]
  15. Tempest D. W., Meers J. L., Brown C. M. Influence of environment on the content and composition of microbial free amino acid pools. J Gen Microbiol. 1970 Dec;64(2):171–185. doi: 10.1099/00221287-64-2-171. [DOI] [PubMed] [Google Scholar]
  16. Trivett T. L., Meyer E. A. Citrate cycle and related metabolism of Listeria monocytogenes. J Bacteriol. 1971 Sep;107(3):770–779. doi: 10.1128/jb.107.3.770-779.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WELSHIMER H. J. VITAMIN REQUIREMENTS OF LISTERIA MONOCYTOGENES. J Bacteriol. 1963 May;85:1156–1159. doi: 10.1128/jb.85.5.1156-1159.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Whatmore A. M., Chudek J. A., Reed R. H. The effects of osmotic upshock on the intracellular solute pools of Bacillus subtilis. J Gen Microbiol. 1990 Dec;136(12):2527–2535. doi: 10.1099/00221287-136-12-2527. [DOI] [PubMed] [Google Scholar]
  19. Whatmore A. M., Reed R. H. Determination of turgor pressure in Bacillus subtilis: a possible role for K+ in turgor regulation. J Gen Microbiol. 1990 Dec;136(12):2521–2526. doi: 10.1099/00221287-136-12-2521. [DOI] [PubMed] [Google Scholar]

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