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. 1992 Dec;58(12):3970–3976. doi: 10.1128/aem.58.12.3970-3976.1992

Production of Pyrroloquinoline Quinone by Using Methanol-Utilizing Bacteria

Teizi Urakami 1,*, Kazuya Yashima 1, Hisao Kobayashi 1, Akio Yoshida 1, Chieko Ito-Yoshida 1
PMCID: PMC183213  PMID: 16348825

Abstract

A large number of methanol-utilizing bacteria were screened for extracellular production of pyrroloquinoline quinone (PQQ) by using methanol as the carbon and energy sources. Of the bacteria selected, Hyphomicrobium sp. strain TK 0441 was examined for PQQ production by using a jar fermentor. The amount of PQQ in the broth and the level of methanol dehydrogenase activity in the cells were increased by simply decreasing the amount of Fe added to the medium. On the other hand, extracellularly produced protein which interfered with the purification of PQQ was decreased by simply increasing the amount of Mg added to the medium. A suitable medium that contained 1 μg of Fe per ml, 150 μg of Mg per ml, and trace elements was developed. In this medium, the production of PQQ reached approximately 1 mg/ml and protein formation was low.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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