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. 1992 Dec;58(12):4051–4054. doi: 10.1128/aem.58.12.4051-4054.1992

Subculturing of a polychlorinated biphenyl-dechlorinating anaerobic enrichment on solid media.

H D May 1, A W Boyle 1, W A Price 2nd 1, C K Blake 1
PMCID: PMC183226  PMID: 1476444

Abstract

An anaerobic culture capable of dechlorinating polychlorinated biphenyls was subcultured under strict anaerobic conditions on solid media containing sterilized river sediment. The dechlorination activity was transferred as a bacterial colony on a solid medium three times. After two transfers on solid medium, the culture was no longer methanogenic but still dechlorinated a mixture of tri- and tetrachlorobiphenyls. This demonstrates that anaerobic bacteria are responsible for the polychlorinated biphenyl dechlorination and can be grown without polychlorinated biphenyl on solid media.

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Selected References

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