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. 1992 Dec;58(12):4060–4063. doi: 10.1128/aem.58.12.4060-4063.1992

Purification and Properties of an Extracellular Agarase from Alteromonas sp. Strain C-1

Oscar Leon 1,*, Luis Quintana 1, Gina Peruzzo 1, Juan Carlos Slebe 1
PMCID: PMC183228  PMID: 16348832

Abstract

A marine bacterial strain isolated from the Bay of San Vicente, Chile, was identified as Alteromonas sp. strain C-1. In the presence of agar, this strain produced high levels of an extracellular agarase. The production of agarase was repressed by glucose, with a parallel decrease in bacterial growth. The enzyme was purified to homogeneity by anion-exchange chromatography and gel filtration, with an overall yield of 45%. The enzyme has a molecular weight of 52,000, is salt sensitive, and hydrolyzes agar, yielding neoagarotetraose as the main product, with an optimum pH of about 6.5.

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Selected References

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