Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 Dec;58(12):4086–4088. doi: 10.1128/aem.58.12.4086-4088.1992

Analysis of exopolysaccharide production by Lactobacillus casei CG11, isolated from cheese.

M Kojic 1, M Vujcic 1, A Banina 1, P Cocconcelli 1, J Cerning 1, L Topisirovic 1
PMCID: PMC183235  PMID: 1476450

Abstract

Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by strain CG11 is linked to a plasmid approximately 30 kb in size.

Full text

PDF
4086

Images in this article

4087Image
on p.4087

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arad S. M., Friedman O. D., Rotem A. Effect of Nitrogen on Polysaccharide Production in a Porphyridium sp. Appl Environ Microbiol. 1988 Oct;54(10):2411–2414. doi: 10.1128/aem.54.10.2411-2414.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bryan B. A., Linhardt R. J., Daniels L. Variation in composition and yield of exopolysaccharides produced by Klebsiella sp. strain K32 and Acinetobacter calcoaceticus BD4. Appl Environ Microbiol. 1986 Jun;51(6):1304–1308. doi: 10.1128/aem.51.6.1304-1308.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cerning J. Exocellular polysaccharides produced by lactic acid bacteria. FEMS Microbiol Rev. 1990 Sep;7(1-2):113–130. doi: 10.1111/j.1574-6968.1990.tb04883.x. [DOI] [PubMed] [Google Scholar]
  5. Morishita T., Deguchi Y., Yajima M., Sakurai T., Yura T. Multiple nutritional requirements of lactobacilli: genetic lesions affecting amino acid biosynthetic pathways. J Bacteriol. 1981 Oct;148(1):64–71. doi: 10.1128/jb.148.1.64-71.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Neve H., Geis A., Teuber M. Plasmid-encoded functions of ropy lactic acid streptococcal strains from Scandinavian fermented milk. Biochimie. 1988 Mar;70(3):437–442. doi: 10.1016/0300-9084(88)90218-0. [DOI] [PubMed] [Google Scholar]
  7. Pidoux M., Marshall V. M., Zanoni P., Brooker B. Lactobacilli isolated from sugary kefir grains capable of polysaccharide production and minicell formation. J Appl Bacteriol. 1990 Sep;69(3):311–320. doi: 10.1111/j.1365-2672.1990.tb01521.x. [DOI] [PubMed] [Google Scholar]
  8. Souw P., Demain A. L. Nutritional Studies on Xanthan Production by Xanthomonas campestris NRRL B1459. Appl Environ Microbiol. 1979 Jun;37(6):1186–1192. doi: 10.1128/aem.37.6.1186-1192.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sutherland I. W. Bacterial exopolysaccharides. Adv Microb Physiol. 1972;8:143–213. doi: 10.1016/s0065-2911(08)60190-3. [DOI] [PubMed] [Google Scholar]
  10. TAYLOR W. H., JUNI E. Pathways for biosynthesis of a bacterial capsular polysaccharide. I. Characterization of the organism and polysaccharide. J Bacteriol. 1961 May;81:688–693. doi: 10.1128/jb.81.5.688-693.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vedamuthu E. R., Neville J. M. Involvement of a Plasmid in Production of Ropiness (Mucoidness) in Milk Cultures by Streptococcus cremoris MS. Appl Environ Microbiol. 1986 Apr;51(4):677–682. doi: 10.1128/aem.51.4.677-682.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. von Wright A., Tynkkynen S. Construction of Streptococcus lactis subsp. lactis Strains with a Single Plasmid Associated with Mucoid Phenotype. Appl Environ Microbiol. 1987 Jun;53(6):1385–1386. doi: 10.1128/aem.53.6.1385-1386.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES