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. 1990 Jan;56(1):167–169. doi: 10.1128/aem.56.1.167-169.1990

Selective plating medium for quantitative recovery of food-borne Listeria monocytogenes.

R V Lachica 1
PMCID: PMC183267  PMID: 2106825

Abstract

A new plating medium (lithium chloride-ceftazidime agar [LCA]) was designed to quantitatively recover food-borne Listeria monocytogenes in the form of large colonies while inhibiting most other food-borne microorganisms. This medium included brain heart infusion agar as the nutritive agar base and a combination of selective agents (LiCl, glycine anhydride, and ceftazidime). Comparison of LCA and lithium chloride-phenylethanol-moxalactam agar (LPM) indicated that both were equally effective for the enumeration of the cold-tolerant pathogen in artificially and naturally contaminated foods. However, LCA was more effective than LPM in the recovery of sublethally heat-injured cells. Moreover, Listeria colonies on LCA exhibited a more distinct bluish hue than those on LPM when viewed by the Henry oblique transillumination technique.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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