Abstract
Two families of peroxidases—lignin peroxidase (LiP) and manganese-dependent lignin peroxidase (MnP)—are formed by the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium and other white rot fungi. Isoenzymes of these enzyme families carry out reactions important to the biodegradation of lignin. This research investigated the regulation of LiP and MnP production by Mn(II). In liquid culture, LiP titers varied as an inverse function of and MnP titers varied as a direct function of the Mn(II) concentration. The extracellular isoenzyme profiles differed radically at low and high Mn(II) levels, whereas other fermentation parameters, including extracellular protein concentrations, the glucose consumption rate, and the accumulation of cell dry weight, did not change significantly with the Mn(II) concentration. In the absence of Mn(II), extracellular LiP isoenzymes predominated, whereas in the presence of Mn(II), MnP isoenzymes were dominant. The release of 14CO2 from 14C-labeled dehydrogenative polymerizate lignin was likewise affected by Mn(II). The rate of 14CO2 release increased at low Mn(II) and decreased at high Mn(II) concentrations. This regulatory effect of Mn(II) occurred with five strains of P. chrysosporium, two other species of Phanerochaete, three species of Phlebia, Lentinula edodes, and Phellinus pini.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andersson L. A., Renganathan V., Chiu A. A., Loehr T. M., Gold M. H. Spectral characterization of diarylpropane oxygenase, a novel peroxide-dependent, lignin-degrading heme enzyme. J Biol Chem. 1985 May 25;260(10):6080–6087. [PubMed] [Google Scholar]
- Forrester I. T., Grabski A. C., Burgess R. R., Leatham G. F. Manganese, Mn-dependent peroxidases, and the biodegradation of lignin. Biochem Biophys Res Commun. 1988 Dec 30;157(3):992–999. doi: 10.1016/s0006-291x(88)80972-0. [DOI] [PubMed] [Google Scholar]
- Glenn J. K., Morgan M. A., Mayfield M. B., Kuwahara M., Gold M. H. An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1983 Aug 12;114(3):1077–1083. doi: 10.1016/0006-291x(83)90672-1. [DOI] [PubMed] [Google Scholar]
- Hammel K. E., Tien M., Kalyanaraman B., Kirk T. K. Mechanism of oxidative C alpha-C beta cleavage of a lignin model dimer by Phanerochaete chrysosporium ligninase. Stoichiometry and involvement of free radicals. J Biol Chem. 1985 Jul 15;260(14):8348–8353. [PubMed] [Google Scholar]
- Jeffries T. W., Choi S., Kirk T. K. Nutritional Regulation of Lignin Degradation by Phanerochaete chrysosporium. Appl Environ Microbiol. 1981 Aug;42(2):290–296. doi: 10.1128/aem.42.2.290-296.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jäger A., Croan S., Kirk T. K. Production of Ligninases and Degradation of Lignin in Agitated Submerged Cultures of Phanerochaete chrysosporium. Appl Environ Microbiol. 1985 Nov;50(5):1274–1278. doi: 10.1128/aem.50.5.1274-1278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kersten P. J., Kirk T. K. Involvement of a new enzyme, glyoxal oxidase, in extracellular H2O2 production by Phanerochaete chrysosporium. J Bacteriol. 1987 May;169(5):2195–2201. doi: 10.1128/jb.169.5.2195-2201.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kersten P. J., Tien M., Kalyanaraman B., Kirk T. K. The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes. J Biol Chem. 1985 Mar 10;260(5):2609–2612. [PubMed] [Google Scholar]
- Keyser P., Kirk T. K., Zeikus J. G. Ligninolytic enzyme system of Phanaerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation. J Bacteriol. 1978 Sep;135(3):790–797. doi: 10.1128/jb.135.3.790-797.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kirk T. K., Connors W. J., Bleam R. D., Hackett W. F., Zeikus J. G. Preparation and microbial decomposition of synthetic [14C]ligins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2515–2519. doi: 10.1073/pnas.72.7.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kirk T. K., Tien M., Kersten P. J., Mozuch M. D., Kalyanaraman B. Ligninase of Phanerochaete chrysosporium. Mechanism of its degradation of the non-phenolic arylglycerol beta-aryl ether substructure of lignin. Biochem J. 1986 May 15;236(1):279–287. doi: 10.1042/bj2360279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niku-Paavola M. L., Karhunen E., Salola P., Raunio V. Ligninolytic enzymes of the white-rot fungus Phlebia radiata. Biochem J. 1988 Sep 15;254(3):877–883. doi: 10.1042/bj2540877. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paszczyński A., Huynh V. B., Crawford R. Comparison of ligninase-I and peroxidase-M2 from the white-rot fungus Phanerochaete chrysosporium. Arch Biochem Biophys. 1986 Feb 1;244(2):750–765. doi: 10.1016/0003-9861(86)90644-2. [DOI] [PubMed] [Google Scholar]
- Reid I. D. Effects of Nitrogen Supplements on Degradation of Aspen Wood Lignin and Carbohydrate Components by Phanerochaete chrysosporium. Appl Environ Microbiol. 1983 Mar;45(3):830–837. doi: 10.1128/aem.45.3.830-837.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tien M., Kirk T. K., Bull C., Fee J. A. Steady-state and transient-state kinetic studies on the oxidation of 3,4-dimethoxybenzyl alcohol catalyzed by the ligninase of Phanerocheate chrysosporium Burds. J Biol Chem. 1986 Feb 5;261(4):1687–1693. [PubMed] [Google Scholar]
- Tien M., Kirk T. K. Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds. Science. 1983 Aug 12;221(4611):661–663. doi: 10.1126/science.221.4611.661. [DOI] [PubMed] [Google Scholar]
- Tien M., Kirk T. K. Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H(2)O(2)-requiring oxygenase. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2280–2284. doi: 10.1073/pnas.81.8.2280. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinstein D. A., Krisnangkura K., Mayfield M. B., Gold M. H. Metabolism of Radiolabeled beta-Guaiacyl Ether-Linked Lignin Dimeric Compounds by Phanerochaete chrysosporium. Appl Environ Microbiol. 1980 Mar;39(3):535–540. doi: 10.1128/aem.39.3.535-540.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]