Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Jan;56(1):230–236. doi: 10.1128/aem.56.1.230-236.1990

Efficiencies of Recovery of Bdellovibrios from Brackish- Water Environments by Using Various Bacterial Species as Prey

A J Schoeffield 1,†, H N Williams 1,*
PMCID: PMC183289  PMID: 16348096

Abstract

A total of 44 bacterial species subdivided into 10 trial experiments have been used as prey for the recovery of bdellovibrios from samples of water from a brackish tidal pond and an aquarium saltwater tank. In an initial investigation, the recovery efficiency of each of the test bacterial species was compared with that of a designated standard prey, Vibrio parahaemolyticus P-5. The results revealed that in each case strain P-5 yielded an equal or significantly greater number of plaques of bdellovibrios than the test prey with but a single exception, strain CS5. In repeat experiments, CS5 yielded fewer plaques than P-5. To determine whether the use of multiple bacterial species compared with a single species as prey would increase the number of PFU of bdellovibrios recovered, material from plaques appearing on each of the test prey in the respective trials was sequentially subcultured onto two respective agar plates, the first containing as prey V. parahaemolyticus P-5 and the second containing the initial test organism. In nearly every case, subculture of plaques from lawns of the test prey to P-5 resulted in plaque formation. On the basis of the results, the use of several test prey and P-5 did not result in the recovery of any more bdellovibrio PFU than the use of P-5 alone. In this study, V. parahaemolyticus P-5 was observed to be the most efficient prey for the recovery of bdellovibrios from moderate salt water.

Full text

PDF
230

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burger A., Drews G., Ladwig R. Wirtskreis und Infektionscyclus eines neu isolierten Bdellovibrio bacteriovorus-Stammes. Arch Mikrobiol. 1968 May 8;61(3):261–279. [PubMed] [Google Scholar]
  2. Enzinger R. M., Cooper R. C. Role of bacteria and protozoa in the removal of Escherichia coli from estuarine waters. Appl Environ Microbiol. 1976 May;31(5):758–763. doi: 10.1128/aem.31.5.758-763.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Houston K. J., Aldridge K. E., Magee L. A. The effect of R antigen on the attachment of Bdellovibrio bacteriovorus to Salmonella typhimurium. Acta Microbiol Pol A. 1974;6(3):253–255. [PubMed] [Google Scholar]
  4. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miyamoto S., Kuroda K. Lethal effect of fresh sea water on Vibrio parahaemolyticus and isolation of Bdellovibrio parasitic against the organism. Jpn J Microbiol. 1975 Aug;19(4):309–317. doi: 10.1111/j.1348-0421.1975.tb00884.x. [DOI] [PubMed] [Google Scholar]
  6. Seidler R. J., Starr M. P. Factors affecting the intracellular parasitic growth of Bdellovibrio bacteriovorus developing within Escherichia coli. J Bacteriol. 1969 Feb;97(2):912–923. doi: 10.1128/jb.97.2.912-923.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Taylor V. I., Baumann P., Reichelt J. L., Allen R. D. Isolation, enumeration, and host range of marine Bdellovibrios. Arch Microbiol. 1974 Jul 4;98(2):101–114. doi: 10.1007/BF00425273. [DOI] [PubMed] [Google Scholar]
  8. Varon M., Shilo M. Attachment of Bdellovibrio bacteriovorus to cell wall mutants of Salmonella spp. and Escherichia coli. J Bacteriol. 1969 Feb;97(2):977–979. doi: 10.1128/jb.97.2.977-979.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Weiner R. M., Hussong D., Colwell R. R. An estuarine agar medium for enumeration of aerobic heterotrophic bacteria associated with water, sediment, and shellfish. Can J Microbiol. 1980 Nov;26(11):1366–1369. doi: 10.1139/m80-226. [DOI] [PubMed] [Google Scholar]
  10. Williams H. N., Falkler W. A., Jr Distribution of bdellovibrios in the water column of an estuary. Can J Microbiol. 1984 Jul;30(7):971–974. doi: 10.1139/m84-152. [DOI] [PubMed] [Google Scholar]
  11. Williams H. N., Falkler W. A., Jr, Shay D. E. Incidence of marine bdellovibrios lytic against Vibrio parahaemolyticus in Chesapeake Bay. Appl Environ Microbiol. 1980 Nov;40(5):970–972. doi: 10.1128/aem.40.5.970-972.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Williams H. N., Falkler W. A., Jr, Shay D. E. Seasonal distribution of bdellovibrios at the mouth of the Patuxent River in the Chesapeake Bay. Can J Microbiol. 1982 Jan;28(1):111–116. doi: 10.1139/m82-011. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES