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Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Jan;56(1):301–303. doi: 10.1128/aem.56.1.301-303.1990

New plate medium for facilitated differentiation of Salmonella spp. from Proteus spp. and other enteric bacteria.

A Rambach 1
PMCID: PMC183329  PMID: 2310184

Abstract

A new agar medium for the differentiation of Salmonella spp. from other members of the family Enterobacteriaceae is described. This medium exploits a novel phenotypic characteristic of Salmonella spp.: the formation of acid from propylene glycol. This characteristic may be used in combination with a chromogenic indicator of beta-galactosidase to differentiate Salmonella spp. from Proteus spp. and the other members of the Enterobacteriaceae. Desoxycholate may be included in the plate medium as an inhibitor of gram-positive organisms. Non-typhi Salmonella spp. yield distinct, bright red colonies on this medium, allowing facilitated identification and unambiguous differentiation from Proteus spp.

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  1. King S., Metzger W. I. A new plating medium for the isolation of enteric pathogens. I. hektoen enteric agar. Appl Microbiol. 1968 Apr;16(4):577–578. doi: 10.1128/am.16.4.577-578.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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