Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Feb;56(2):401–408. doi: 10.1128/aem.56.2.401-408.1990

Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm.

J T Trevors 1, J D van Elsas 1, L S van Overbeek 1, M E Starodub 1
PMCID: PMC183352  PMID: 2106286

Abstract

Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed with groundwater. The presence of wheat roots growing downward in the microcosms contributed only slightly to the movement of P. fluorescens C5t cells to lower soil regions of the loamy sand microcosms, but enhanced downward transport in the loam microcosms. Furthermore, the introduced P. fluorescens C5t cells were detected in the effluent water samples even after three flushes of groundwater and 10 days of incubation. As evidenced by a comparison of counts from immunofluorescence and selective plating, nonculturable C5t cells occurred in day 10 soil and percolated water samples, primarily of the loamy sand microcosms. Vertical soil microcosms that use water movement may be useful in studying the survival and transport of genetically engineered bacteria in soil under a variety of conditions prior to field testing.

Full text

PDF
401

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Compeau G., Al-Achi B. J., Platsouka E., Levy S. B. Survival of rifampin-resistant mutants of Pseudomonas fluorescens and Pseudomonas putida in soil systems. Appl Environ Microbiol. 1988 Oct;54(10):2432–2438. doi: 10.1128/aem.54.10.2432-2438.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Festl H., Ludwig W., Schleifer K. H. DNA hybridization probe for the Pseudomonas fluorescens group. Appl Environ Microbiol. 1986 Nov;52(5):1190–1194. doi: 10.1128/aem.52.5.1190-1194.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Griffin D. M., Quail G. Movement of bacteria in moist, particulate systems. Aust J Biol Sci. 1968 Jun;21(3):579–582. doi: 10.1071/bi9680579. [DOI] [PubMed] [Google Scholar]
  4. Gurijala K. R., Alexander M. Role of sublethal injury in decline of bacterial populations in lake water. Appl Environ Microbiol. 1988 Nov;54(11):2859–2861. doi: 10.1128/aem.54.11.2859-2861.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hartel P. G., Billingsley J. W., Williamson J. W. Styrofoam cup-membrane assembly for studying microorganism-root interactions. Appl Environ Microbiol. 1989 May;55(5):1291–1294. doi: 10.1128/aem.55.5.1291-1294.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holben William E., Jansson Janet K., Chelm Barry K., Tiedje James M. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community. Appl Environ Microbiol. 1988 Mar;54(3):703–711. doi: 10.1128/aem.54.3.703-711.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schaub S. A., Sorber C. A. Virus and bacteria removal from wastewater by rapid infiltration through soil. Appl Environ Microbiol. 1977 Mar;33(3):609–619. doi: 10.1128/aem.33.3.609-619.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Soby S., Bergman K. Motility and Chemotaxis of Rhizobium meliloti in Soil. Appl Environ Microbiol. 1983 Nov;46(5):995–998. doi: 10.1128/aem.46.5.995-998.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Steffan R. J., Atlas R. M. DNA amplification to enhance detection of genetically engineered bacteria in environmental samples. Appl Environ Microbiol. 1988 Sep;54(9):2185–2191. doi: 10.1128/aem.54.9.2185-2191.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Trevors J. T., Oddie K. M. R-plasmid transfer in soil and water. Can J Microbiol. 1986 Jul;32(7):610–613. doi: 10.1139/m86-114. [DOI] [PubMed] [Google Scholar]
  11. Trevors J. T., van Elsas J. D., Starodub M. E., Van Overbeek L. S. Survival of and plasmid stability in Pseudomonas and Klebsiella spp. introduced into agricultural drainage water. Can J Microbiol. 1989 Jul;35(7):675–680. doi: 10.1139/m89-110. [DOI] [PubMed] [Google Scholar]
  12. Weller D. M. Distribution of a Take-All Suppressive Strain of Pseudomonas fluorescens on Seminal Roots of Winter Wheat. Appl Environ Microbiol. 1984 Oct;48(4):897–899. doi: 10.1128/aem.48.4.897-899.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES