Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Mar;56(3):808–811. doi: 10.1128/aem.56.3.808-811.1990

Monoclonal antibody to type F Clostridium botulinum toxin.

J L Ferreira 1, M K Hamdy 1, S G McCay 1, F A Zapatka 1
PMCID: PMC183425  PMID: 2180373

Abstract

Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.

Full text

PDF
808

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boroff D. A., Chu-Chen G. Radioimmunoassay for type A toxin of Clostridium botulinum. Appl Microbiol. 1973 Apr;25(4):545–549. doi: 10.1128/am.25.4.545-549.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ferreira J. L., Hamdy M. K., Herd Z. L., McCay S. G., Zapatka F. A. Monoclonal antibody for the detection of Clostridium botulinum type A toxin. Mol Cell Probes. 1987 Dec;1(4):337–345. doi: 10.1016/0890-8508(87)90015-6. [DOI] [PubMed] [Google Scholar]
  3. Kozaki S., Kamata Y., Nagai T., Ogasawara J., Sakaguchi G. The use of monoclonal antibodies to analyze the structure of Clostridium botulinum type E derivative toxin. Infect Immun. 1986 Jun;52(3):786–791. doi: 10.1128/iai.52.3.786-791.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MOLLER V., SCHEIBEL I. Preliminary report on the isolation of an apparently new type of CI. botulinum. Acta Pathol Microbiol Scand. 1960;48:80–80. doi: 10.1111/j.1699-0463.1960.tb04741.x. [DOI] [PubMed] [Google Scholar]
  5. Notermans S., Hagenaars A. M., Kozaki S. The enzyme-linked immunosorbent assay (ELISA) for the detection and determination of Clostridium botulinum toxins A, B, and E. Methods Enzymol. 1982;84:223–238. doi: 10.1016/0076-6879(82)84020-2. [DOI] [PubMed] [Google Scholar]
  6. Oguma K., Agui T., Syuto B., Kimura K., Iida H., Kubo S. Four different monoclonal antibodies against type C1 toxin of Clostridium botulinum. Infect Immun. 1982 Oct;38(1):14–20. doi: 10.1128/iai.38.1.14-20.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tsuzuki K., Yokosawa N., Syuto B., Ohishi I., Fujii N., Kimura K., Oguma K. Establishment of a monoclonal antibody recognizing an antigenic site common to Clostridium botulinum type B, C1, D, and E toxins and tetanus toxin. Infect Immun. 1988 Apr;56(4):898–902. doi: 10.1128/iai.56.4.898-902.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Vermilyea B. L., Walker H. W., Ayres J. C. Detection of botulinal toxins by immunodiffusion. Appl Microbiol. 1968 Jan;16(1):21–24. doi: 10.1128/am.16.1.21-24.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yang K. H., Sugiyama H. Purification and properties of Clostridium botulinum type F toxin. Appl Microbiol. 1975 May;29(5):598–603. doi: 10.1128/am.29.5.598-603.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES