Abstract
Barophilic bacteria are microorganisms that grow preferentially (facultative barophiles) or exclusively (obligate barophiles) under elevated hydrostatic pressure. Barophilic bacteria have been isolated from a variety of deep-sea environments. Attempts to characterize these organisms have been hampered by a lack of appropriate methodologies. A colorimetric method for the detection of 19 constitutively expressed enzymes under in situ conditions of pressure and temperature has been devised, using a simple modification of the commercially available API ZYME enzyme assay kit. By using this method, enzyme profiles of 11 barophilic isolates, including an obligate barophile, were determined. Nine of the 10 facultatively barophilic isolates examined exhibited a change of phenotype in at least one enzyme reaction when tested at 1 atm (1 atm = 101.29 kPa), compared with results obtained under in situ pressure. The assay is simple and rapid and allows for direct determination of enzyme activity under conditions of high pressure and low temperature.
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- D'Amato R. F., Eriquez L. A., Tomfohrde K. M., Singerman E. Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles. J Clin Microbiol. 1978 Jan;7(1):77–81. doi: 10.1128/jcm.7.1.77-81.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Delong E. F., Yayanos A. A. Properties of the glucose transport system in some deep-sea bacteria. Appl Environ Microbiol. 1987 Mar;53(3):527–532. doi: 10.1128/aem.53.3.527-532.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deming J. W., Hada H., Colwell R. R., Luehrsen K. R., Fox G. E. The ribonucleotide sequence of 5s rRNA from two strains of deep-sea barophilic bacteria. J Gen Microbiol. 1984 Aug;130(8):1911–1920. doi: 10.1099/00221287-130-8-1911. [DOI] [PubMed] [Google Scholar]
- Humble M. W., King A., Phillips I. API ZYM: a simple rapid system for the detection of bacterial enzymes. J Clin Pathol. 1977 Mar;30(3):275–277. doi: 10.1136/jcp.30.3.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morita R. Y. Microbial life in the deep sea. Can J Microbiol. 1980 Dec;26(12):1375–1385. doi: 10.1139/m80-230. [DOI] [PubMed] [Google Scholar]
- Nolte F. S., Hollick G. E., Robertson R. G. Enzymatic activities of Legionella pneumophila and Legionella-like organisms. J Clin Microbiol. 1982 Jan;15(1):175–177. doi: 10.1128/jcm.15.1.175-177.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Brien M., Davis G. H. Enzymatic profile of Pseudomonas maltophilia. J Clin Microbiol. 1982 Sep;16(3):417–421. doi: 10.1128/jcm.16.3.417-421.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slots J. Enzymatic characterization of some oral and nonoral gram-negative bacteria with the API ZYM system. J Clin Microbiol. 1981 Sep;14(3):288–294. doi: 10.1128/jcm.14.3.288-294.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tharagonnet D., Sisson P. R., Roxby C. M., Ingham H. R., Selkon J. B. The API ZYM system in the identification of Gram-negative anaerobes. J Clin Pathol. 1977 Jun;30(6):505–509. doi: 10.1136/jcp.30.6.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yayanos A. A., Dietz A. S., Van Boxtel R. Obligately barophilic bacterium from the Mariana trench. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5212–5215. doi: 10.1073/pnas.78.8.5212. [DOI] [PMC free article] [PubMed] [Google Scholar]