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. 1991 Jun;57(6):1595–1601. doi: 10.1128/aem.57.6.1595-1601.1991

Characterization of exopolymers of aquatic bacteria by pyrolysis-mass spectrometry.

T Ford 1, E Sacco 1, J Black 1, T Kelley 1, R Goodacre 1, R C Berkeley 1, R Mitchell 1
PMCID: PMC183438  PMID: 11536484

Abstract

Exopolymers from a diverse collection of marine and freshwater bacteria were characterized by pyrolysis-mass spectrometry (Py-MS). Py-MS provides spectra of pyrolysis fragments that are characteristic of the original material. Analysis of the spectra by multivariate statistical techniques (principal component and canonical variate analysis) separated these exopolymers into distinct groups. Py-MS clearly distinguished characteristic fragments, which may be derived from components responsible for functional differences between polymers. The importance of these distinctions and the relevance of pyrolysis information to exopolysaccharide function in aquatic bacteria is discussed.

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Selected References

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