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. 1991 Jun;57(6):1602–1608. doi: 10.1128/aem.57.6.1602-1608.1991

Performance characterization of a model bioreactor for the biodegradation of trichloroethylene by Pseudomonas cepacia G4.

B R Folsom 1, P J Chapman 1
PMCID: PMC183439  PMID: 1872599

Abstract

Pseudomonas cepacia G4 grown in chemostats with phenol demonstrated constant specific degradation rates for both phenol and trichloroethylene (TCE) over a range of dilution rates. Washout of cells from chemostats was evident at a dilution rate of 0.2 h-1 at 28 degrees C. Increased phenol concentrations in the nutrient feed led to increased biomass production with constant specific degradation rates for both phenol and TCE. The addition of lactate to the phenol feed led to increased biomass production but lowered specific phenol and TCE degradation rates. The maximum potential for TCE degradation was about 1.1 g per day per g of cell protein. Cell growth and degradation kinetic parameters were used in the design of a recirculating bioreactor for TCE degradation. In this reactor, the total amount of TCE degraded increased as either reaction time or biomass was increased. TCE degradation was observed up to 300 microM TCE with no significant decreases in rates. On the average, this reactor was able to degrade 0.7 g of TCE per day per g of cell protein. These results demonstrate the feasibility of TCE bioremediation through the use of bioreactors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arciero D., Vannelli T., Logan M., Hooper A. B. Degradation of trichloroethylene by the ammonia-oxidizing bacterium Nitrosomonas europaea. Biochem Biophys Res Commun. 1989 Mar 15;159(2):640–643. doi: 10.1016/0006-291x(89)90042-9. [DOI] [PubMed] [Google Scholar]
  2. Bouwer E. J., McCarty P. L. Transformations of 1- and 2-carbon halogenated aliphatic organic compounds under methanogenic conditions. Appl Environ Microbiol. 1983 Apr;45(4):1286–1294. doi: 10.1128/aem.45.4.1286-1294.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Folsom B. R., Chapman P. J., Pritchard P. H. Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates. Appl Environ Microbiol. 1990 May;56(5):1279–1285. doi: 10.1128/aem.56.5.1279-1285.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hareland W. A., Crawford R. L., Chapman P. J., Dagley S. Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. J Bacteriol. 1975 Jan;121(1):272–285. doi: 10.1128/jb.121.1.272-285.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nelson M. J., Montgomery S. O., Mahaffey W. R., Pritchard P. H. Biodegradation of trichloroethylene and involvement of an aromatic biodegradative pathway. Appl Environ Microbiol. 1987 May;53(5):949–954. doi: 10.1128/aem.53.5.949-954.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nelson M. J., Montgomery S. O., O'neill E. J., Pritchard P. H. Aerobic metabolism of trichloroethylene by a bacterial isolate. Appl Environ Microbiol. 1986 Aug;52(2):383–384. doi: 10.1128/aem.52.2.383-384.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oldenhuis R., Vink R. L., Janssen D. B., Witholt B. Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase. Appl Environ Microbiol. 1989 Nov;55(11):2819–2826. doi: 10.1128/aem.55.11.2819-2826.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Phelps T. J., Niedzielski J. J., Schram R. M., Herbes S. E., White D. C. Biodegradation of trichloroethylene in continuous-recycle expanded-bed bioreactors. Appl Environ Microbiol. 1990 Jun;56(6):1702–1709. doi: 10.1128/aem.56.6.1702-1709.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pritchard J. Alberta. Potomac horse fever. Can Vet J. 1989 Mar;30(3):256–256. [PMC free article] [PubMed] [Google Scholar]
  10. Shields M. S., Montgomery S. O., Chapman P. J., Cuskey S. M., Pritchard P. H. Novel pathway of toluene catabolism in the trichloroethylene-degrading bacterium g4. Appl Environ Microbiol. 1989 Jun;55(6):1624–1629. doi: 10.1128/aem.55.6.1624-1629.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tsien H. C., Brusseau G. A., Hanson R. S., Waclett L. P. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b. Appl Environ Microbiol. 1989 Dec;55(12):3155–3161. doi: 10.1128/aem.55.12.3155-3161.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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