Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Jun;57(6):1624–1629. doi: 10.1128/aem.57.6.1624-1629.1991

Typing of human, animal, food, and environmental isolates of Listeria monocytogenes by multilocus enzyme electrophoresis.

P Boerlin 1, J C Piffaretti 1
PMCID: PMC183442  PMID: 1908204

Abstract

In order to elucidate some aspects of the epidemiology of listeriosis in Switzerland, 181 strains of Listeria monocytogenes isolated from humans, animals, food, and the environment have been analyzed by multilocus enzyme electrophoresis at 21 enzyme loci. The clone responsible for several recent food-borne outbreaks in Switzerland and in North America (marked by electrophoretic type 1 and serovar 4b) has been found frequently among strains isolated from animals. Thus, animals may represent a major source of diffusion of this clone in the environment and in food, in which it has been found only sporadically, however. Two other unrelated clones (including strains belonging to serovars 1/2b and 1/2c) have often been isolated from meat but not from animals. These findings indicate that contamination of meat with L. monocytogenes might originate mainly from the environment in which it is processed rather than from animals themselves. This could explain the differences in the distribution of L. monocytogenes serovars isolated from meat and from animals.

Full text

PDF
1624

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Audurier A., Martin C. Phage typing of Listeria monocytogenes. Int J Food Microbiol. 1989 Jun;8(3):251–257. doi: 10.1016/0168-1605(89)90022-6. [DOI] [PubMed] [Google Scholar]
  2. Bannister B. A. Listeria monocytogenes meningitis associated with eating soft cheese. J Infect. 1987 Sep;15(2):165–168. doi: 10.1016/s0163-4453(87)93181-1. [DOI] [PubMed] [Google Scholar]
  3. Bibb W. F., Gellin B. G., Weaver R., Schwartz B., Plikaytis B. D., Reeves M. W., Pinner R. W., Broome C. V. Analysis of clinical and food-borne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations. Appl Environ Microbiol. 1990 Jul;56(7):2133–2141. doi: 10.1128/aem.56.7.2133-2141.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bibb W. F., Schwartz B., Gellin B. G., Plikaytis B. D., Weaver R. E. Analysis of Listeria monocytogenes by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations. Int J Food Microbiol. 1989 Jun;8(3):233–239. doi: 10.1016/0168-1605(89)90018-4. [DOI] [PubMed] [Google Scholar]
  5. Boerlin P., Rocourt J., Piffaretti J. C. Taxonomy of the genus Listeria by using multilocus enzyme electrophoresis. Int J Syst Bacteriol. 1991 Jan;41(1):59–64. doi: 10.1099/00207713-41-1-59. [DOI] [PubMed] [Google Scholar]
  6. Farber J. M., Carter A. O., Varughese P. V., Ashton F. E., Ewan E. P. Listeriosis traced to the consumption of alfalfa tablets and soft cheese. N Engl J Med. 1990 Feb 1;322(5):338–338. doi: 10.1056/nejm199002013220519. [DOI] [PubMed] [Google Scholar]
  7. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  8. Gray M. L., Killinger A. H. Listeria monocytogenes and listeric infections. Bacteriol Rev. 1966 Jun;30(2):309–382. doi: 10.1128/br.30.2.309-382.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ho J. L., Shands K. N., Friedland G., Eckind P., Fraser D. W. An outbreak of type 4b Listeria monocytogenes infection involving patients from eight Boston hospitals. Arch Intern Med. 1986 Mar;146(3):520–524. [PubMed] [Google Scholar]
  10. Kaczmarski E. B., Jones D. M. Listeriosis and ready-cooked chicken. Lancet. 1989 Mar 11;1(8637):549–549. doi: 10.1016/s0140-6736(89)90084-6. [DOI] [PubMed] [Google Scholar]
  11. Kampelmacher E. H., van Noorle Jansen L. M. Listeriosis in humans and animals in the Netherlands (1958-1977). Zentralbl Bakteriol A. 1980 Mar;246(2):211–227. [PubMed] [Google Scholar]
  12. Lennon D., Lewis B., Mantell C., Becroft D., Dove B., Farmer K., Tonkin S., Yeates N., Stamp R., Mickleson K. Epidemic perinatal listeriosis. Pediatr Infect Dis. 1984 Jan-Feb;3(1):30–34. doi: 10.1097/00006454-198401000-00008. [DOI] [PubMed] [Google Scholar]
  13. Linnan M. J., Mascola L., Lou X. D., Goulet V., May S., Salminen C., Hird D. W., Yonekura M. L., Hayes P., Weaver R. Epidemic listeriosis associated with Mexican-style cheese. N Engl J Med. 1988 Sep 29;319(13):823–828. doi: 10.1056/NEJM198809293191303. [DOI] [PubMed] [Google Scholar]
  14. McLauchlin J. Distribution of serovars of Listeria monocytogenes isolated from different categories of patients with listeriosis. Eur J Clin Microbiol Infect Dis. 1990 Mar;9(3):210–213. doi: 10.1007/BF01963840. [DOI] [PubMed] [Google Scholar]
  15. McLauchlin J., Greenwood M. H., Pini P. N. The occurrence of Listeria monocytogenes in cheese from a manufacturer associated with a case of listeriosis. Int J Food Microbiol. 1990 May;10(3-4):255–262. doi: 10.1016/0168-1605(90)90073-e. [DOI] [PubMed] [Google Scholar]
  16. McLauchlin J. Listeria monocytogenes, recent advances in the taxonomy and epidemiology of listeriosis in humans. J Appl Bacteriol. 1987 Jul;63(1):1–11. doi: 10.1111/j.1365-2672.1987.tb02411.x. [DOI] [PubMed] [Google Scholar]
  17. Piffaretti J. C., Kressebuch H., Aeschbacher M., Bille J., Bannerman E., Musser J. M., Selander R. K., Rocourt J. Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease. Proc Natl Acad Sci U S A. 1989 May;86(10):3818–3822. doi: 10.1073/pnas.86.10.3818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rocourt J., Seeliger H. P. Distribution des espèces du genre Listeria. Zentralbl Bakteriol Mikrobiol Hyg A. 1985 May;259(3):317–330. [PubMed] [Google Scholar]
  19. Schlech W. F., 3rd, Lavigne P. M., Bortolussi R. A., Allen A. C., Haldane E. V., Wort A. J., Hightower A. W., Johnson S. E., King S. H., Nicholls E. S. Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983 Jan 27;308(4):203–206. doi: 10.1056/NEJM198301273080407. [DOI] [PubMed] [Google Scholar]
  20. Schwartz B., Ciesielski C. A., Broome C. V., Gaventa S., Brown G. R., Gellin B. G., Hightower A. W., Mascola L. Association of sporadic listeriosis with consumption of uncooked hot dogs and undercooked chicken. Lancet. 1988 Oct 1;2(8614):779–782. doi: 10.1016/s0140-6736(88)92425-7. [DOI] [PubMed] [Google Scholar]
  21. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Skovgaard N., Morgen C. A. Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. Int J Food Microbiol. 1988 May;6(3):229–242. doi: 10.1016/0168-1605(88)90015-3. [DOI] [PubMed] [Google Scholar]
  23. Skovgaard N., Nørrung B. The incidence of Listeria spp. in faeces of Danish pigs and in minced pork meat. Int J Food Microbiol. 1989 Feb;8(1):59–63. doi: 10.1016/0168-1605(89)90080-9. [DOI] [PubMed] [Google Scholar]
  24. Weis J., Seeliger H. P. Incidence of Listeria monocytogenes in nature. Appl Microbiol. 1975 Jul;30(1):29–32. doi: 10.1128/am.30.1.29-32.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES