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. 1991 Jun;57(6):1838–1841. doi: 10.1128/aem.57.6.1838-1841.1991

Molecular structure of the replication origin of a Bacillus subtilis (natto) plasmid, pUH1.

T Hara 1, S Nagatomo 1, S Ogata 1, S Ueda 1
PMCID: PMC183478  PMID: 1840479

Abstract

The structure of a 2.0-kb BstEII DNA sequence necessary and sufficient for the replication of a 5.7-kb Natto plasmid, pUH1, which is responsible for gamma-polyglutamate production by Bacillus subtilis (natto), has been characterized by using a trimethoprim resistance gene derived from B. subtilis chromosomal DNA as a selective marker. The 2.0-kb DNA sequence contains an open reading frame, rep, stretching for 999 bp; a promoter region for rep expression; and a possible replication origin for the plasmid upstream of the promotor. The predicted Rep protein has highly homologous amino acid sequences with rep14 of pFTB14 in B. amyloliquefaciens, RepB of pUB110, and protein A, which is necessary for pC194 replication in staphylococci throughout the protein molecule, but is not homologous with RepC of staphylococcal plasmid pT181.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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