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. 1991 Jun;57(6):1870–1872. doi: 10.1128/aem.57.6.1870-1872.1991

Cloning of alginate lyase gene (alxM) and expression in Escherichia coli.

B J Brown 1, J F Preston 1, L O Ingram 1
PMCID: PMC183488  PMID: 1872617

Abstract

The alxM gene encoding a D-mannuronan-specific alginate lyase has been cloned from a marine bacterium isolated as an epiphyte on the brown alga, Sargassum fluitans. Expression of this gene in Escherichia coli provides a source of this enzyme for probing alginate structure and modifying the mannuronan-rich alginate polymers produced by bacterial pathogens.

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Selected References

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