Abstract
The anaerobic growth and fermentation of a marine isolate of Paecilomyces lilacinus is described. The fungus was isolated from mullet gut and grew optimally at 30°C and at a salinity of ≥10%. The best growth was obtained with glucose or laminarin as substrate, and the growth yield was 5.0 g (dry weight of fungus) per mol of hexose fermented. Moles of products as a percentage of moles of hexose fermented were acetate, 29.0%; ethanol, 156.6%; CO2, 108.0%; and lactate, 4.3%. Together these products accounted for >80% of hexose carbon. Hydrogen and formate were not detectable as fermentation end products (<0.5%). Other substrates utilized for growth, although less effectively than laminarin or glucose, included the monosaccharides galactose, fructose, arabinose, and xylose and the disaccharides maltose and cellobiose. No growth of the fungus occurred on cellulose, and of a variety of other polysaccharides tested only xylan supported growth.
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- BAILEY R. W. The reaction of pentoses with anthrone. Biochem J. 1958 Apr;68(4):669–672. doi: 10.1042/bj0680669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bauchop T., Mountfort D. O. Cellulose fermentation by a rumen anaerobic fungus in both the absence and the presence of rumen methanogens. Appl Environ Microbiol. 1981 Dec;42(6):1103–1110. doi: 10.1128/aem.42.6.1103-1110.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bryant M. P. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1324–1328. doi: 10.1093/ajcn/25.12.1324. [DOI] [PubMed] [Google Scholar]
- Duckworth M., Turvey J. R. An extracellular agarase from a Cytophaga species. Biochem J. 1969 Jun;113(1):139–142. doi: 10.1042/bj1130139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUGGETT A. S., NIXON D. A. Use of glucose oxidase, peroxidase, and O-dianisidine in determination of blood and urinary glucose. Lancet. 1957 Aug 24;273(6991):368–370. doi: 10.1016/s0140-6736(57)92595-3. [DOI] [PubMed] [Google Scholar]
- Tangarone B., Royer J. C., Nakas J. P. Purification and Characterization of an Endo-(1,3)-beta-d-Glucanase from Trichoderma longibrachiatum. Appl Environ Microbiol. 1989 Jan;55(1):177–184. doi: 10.1128/aem.55.1.177-184.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YAPHE W., MORGAN K. Enzymic hydrolysis of fucoidin by Pseudomonas atlantica and Pseudomonas carrageenovora. Nature. 1959 Mar 14;183(4663):761–762. doi: 10.1038/183761b0. [DOI] [PubMed] [Google Scholar]