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. 1991 Jul;57(7):2027–2032. doi: 10.1128/aem.57.7.2027-2032.1991

Use of an A-T-rich DNA clone for identification and detection of Peronosclerospora sorghi.

C L Yao 1, C W Magill 1, R A Frederiksen 1
PMCID: PMC183516  PMID: 1892392

Abstract

A recombinant plasmid, pMLY12-1, screened from a Peronosclerospora sorghi library hybridizes only to DNA of P. sorghi, or to DNA from leaves infected with P. sorghi, not to DNA of P. sorghi Thailand isolate, P. philippinensis, P. sacchari, or P. maydis. The terminal sequences of the 1.3-kb insert, which appears to contain mitochondrial DNA, are 85% A and T. No polymorphisms were detected when the probe was hybridized to Southern blots containing DNA from P. sorghi pathotype 1, pathotype 3, or a Botswana isolate digested with any of the eight restriction endonucleases tested. The banding patterns were the same whether DNA was extracted directly from the fungus or from infected leaves.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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